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RASL10A

Plasmid

#55674

Purpose

expression of mouse RASL10A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL10A (Rasl10a Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
RHEBL1

Plasmid

#55684

Purpose

expression of mouse RHEBL1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RHEBL1 (Rhebl1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
flGalT-SBP-mCherry_puromycin

Plasmid

#65276

Purpose

GalT reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Flag‐Tim2

Plasmid

#49213

Purpose

expresses Flag tagged Tim2

Depositor

Lawrence Kane

Article

Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

Insert

Tim2 (Timd2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

no Tim2 signal sequence; R80L and S196D compared …

Promoter

EF1a

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only
RASL11B

Plasmid

#55678

Purpose

expression of mouse RASL11B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL11B (Rasl11b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
RASL10B

Plasmid

#55673

Purpose

expression of mouse RASL10B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL10B (Rasl10b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pProUSER13F4C

Plasmid

#178023

Purpose

E. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanA

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Pman

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13E3F

Plasmid

#178026

Purpose

E. coli - B. subtilis nicking vector pProUSER13E3F. Ap-BBR1; yhgE-insertion-site; Spc; tetR-Ptet

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Ptet

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13G2F

Plasmid

#178027

Purpose

E. coli - B. subtilis nicking vector pProUSER13G2F . Ap-BBR1; rsbP-insertion-site; Ery; tetR-Ptet

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Ptet

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pTarget-Dual

Plasmid

#207884

Purpose

Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Inserts

I-OnuI target site
I-GpeMI target site

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV hSyn mRuby

Plasmid

#220912

Purpose

Neuronal specific expression of mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

mRuby

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCitro-15a

Plasmid

#202033

Purpose

p15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter

Depositor

David Edgell

Article

Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

Insert

conjugative system from a Citrobacter spp.

Use

Synthetic Biology

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-TC9

Plasmid

#202088

Purpose

entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

Depositor

David Edgell

Article

Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

Insert

TevCas9 + sgRNA cassette

Use

CRISPR

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 T3550A/S3556A

Plasmid

#199572

Purpose

Expresses mutated SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-4 β4

Plasmid

#198327

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 β4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Contains L480S substitution

Promoter

ADH1

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1C

Plasmid

#198336

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1C

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

S148C substitution

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-AP-3 σ3A

Plasmid

#198347

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-3 σ3A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-AP-3 σ3B

Plasmid

#198348

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-3 σ3B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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