We narrowed to 10,664 results for: t7

1. pET15T-MsePCNA3

   Plasmid

   #107065

   Purpose

   Expresses M. sedula PCNA3 in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   M. sedula PCNA3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJExpress414_GmPOPB-R663K

   Plasmid

   #92239

   Purpose

   Express R663K mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-R663K mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Arginine 663 to Lysine

   Promoter

   T7 promoter

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAK22

   Plasmid

   #101741

   Purpose

   encoding two copies of T. thermophilus SecG for expression of SecYEG complex

   Depositor

   Tomoya Tsukazaki

   Article

   Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. do

   Insert

   Thermus thermophilus HB8 SecG x 2
   

   Tags

   NONE

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJExpress414_GmPOPB-W695del

   Plasmid

   #92241

   Purpose

   Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Tryptophan 695 deleted

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJExpress414_GmPOPB-R663Q

   Plasmid

   #92240

   Purpose

   Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-R663Q mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Arginine 663 to Glutamine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJExpress414_GmPOPB-H698A

   Plasmid

   #92237

   Purpose

   Express H698A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-H698A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Histidine 698 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJExpress414_GmPOPB-R663A

   Plasmid

   #92238

   Purpose

   Express R663A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-R663A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Arginine 663 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. JPUB_006820

   Plasmid

   #87921

   Purpose

   E. coli expression of K. stuttgartiensis protein

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Insert

   Kuste2803
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. JPUB_006826

   Plasmid

   #87923

   Purpose

   E. coli expression of K. stuttgartiensis protein

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Insert

   Kuste3608
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. JPUB_006834

    Plasmid

    #87920

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste3608
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. JPUB_006828

    Plasmid

    #87922

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste2803
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MetNI (E166Q in MetN)

    Plasmid

    #78320

    Purpose

    Methionine ABC Transporter

    Depositor

    Douglas Rees

    Article

    Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

    Inserts

    MetI
    MetN
    

    Tags

    10x Histidine Tag and 6x Histidine Tag

    Expression

    Bacterial

    Mutation

    E166Q

    Promoter

    T7 Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-Chaetomium thermophilum LIC

    Plasmid

    #74782

    Purpose

    encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Chaetomium thermophilum dynein light intermediate chain
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK044 (pET-PurQ-wt)

    Plasmid

    #73839

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK560

    Plasmid

    #71585

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Expression

    Bacterial

    Mutation

    changed arginine-232 to histidine

    Promoter

    T7 promoter

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK504

    Plasmid

    #71584

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-357 to tyrosine

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK613

    Plasmid

    #72534

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    residues 1-69 only; C-terminal domain removed and…

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMPO 62

    Plasmid

    #69870

    Purpose

    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII- sspH2 signal peptide, M13 replicat

    Depositor

    Eduardo Santero

    Article

    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK136

    Plasmid

    #71122

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK376

    Plasmid

    #70235

    Purpose

    Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE)

    Depositor

    T. J. Kappock

    Article

    Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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