We narrowed to 9,933 results for: Uty

1. pCVL.SSA TLR (Sce)

   Plasmid

   #45576

   Purpose

   Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with I-Sce I TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185bp, truncated 25 …

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvol-pAzFRS.1.t1

   Plasmid

   #73547

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.1.t1
   pAzFRS.1.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB1 (CK2alpha)

   Plasmid

   #27083

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pCAG eGFP T2A SP HALO HAPLN1

   Plasmid

   #228200

   Purpose

   AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

   Depositor

   Sungjin Park

   Article

   Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

   Insert

   HAPLN1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   constitutive CAG promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 TadCBEd-V106W

   Plasmid

   #193839

   Purpose

   Express TadCBEd-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd V106W-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-pmExRai-AKAR2

   Plasmid

   #161755

   Purpose

   Plasma membrane-targeted ExRai-AKAR2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   Lyn-ExRai-AKAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-SPOTlight-U2GCR

   Plasmid

   #164819

   Purpose

   Reporter to measure ISR state-dependent protein synthesis

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   SPOTlight-U2GCR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYTRW09K_0T5

   Plasmid

   #177281

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   Ptet-tetA(C)
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mitoExRai-AKAR2

   Plasmid

   #161757

   Purpose

   ExRai-AKAR2 targeted to the outer mitochondrial membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   DAKAP-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME map2k2a_s219D

    Plasmid

    #201058

    Purpose

    Gateway middle entry vector expressing constitutively active zebrafish map2k2a

    Depositor

    Sarah Childs

    Article

    Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

    Insert

    MAP2k2a (map2k2a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Mutation S219D, constitutively active

    Promoter

    none

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Prig-3::CD4::spGFP1-10

    Plasmid

    #65829

    Purpose

    For membrane contact

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
    

    Use

    Tags

    spGFP1-10

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Prig-3::CD4::spGFP11

    Plasmid

    #65830

    Purpose

    For membrane contact

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
    

    Use

    Tags

    spGFP11

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHiUGE smFP-V5 donor (all-in-one) ORF2

    Plasmid

    #200388

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPalmitoyl-mTurquoise2

    Plasmid

    #36209

    Purpose

    In vivo visualization of the membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    p63(1-29) (ARHGEF25 Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-erExRai-AKAR2

    Plasmid

    #161756

    Purpose

    ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    CYP450-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNCST-AvicFP1

    Plasmid

    #129504

    Purpose

    Expresses AvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNCST-AvicFP2

    Plasmid

    #129505

    Purpose

    Expresses AvicFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNCST-AvicFP3

    Plasmid

    #129506

    Purpose

    Expresses AvicFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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