We narrowed to 5,960 results for: actin

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pCB004

Plasmid

#134147

Purpose

Bacterial expression of human Ska3

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

human Ska3 (SKA3 Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1-wt Fzd3 3'UTR

Plasmid

#25024

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

wt Fzd3 3'UTR

Use

Luciferase

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pgRNAtet-phaZ

Plasmid

#169874

Purpose

Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards phaZ in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pNVLTv2-pvdIJD-Prha-base

Plasmid

#169244

Purpose

Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

rhaRS

Expression

Bacterial

Promoter

rhaS

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-HIPK4

Plasmid

#23760

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HIPK4 (HIPK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
His-ZZ-TEV-BICDR1

Plasmid

#111859

Purpose

Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Tags

His-tag, TEV site, and ZZ

Expression

Insect

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pJS647

Plasmid

#183176

Purpose

PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Bacterial

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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His-NKX3.1

Plasmid

#42896

Depositor

Edward Gelmann

Article

Ju et al Biochemistry. 2009 Nov 10;48(44):10601-7

Insert

NKX3.1 (NKX3-1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-Poly(A)

Plasmid

#50556

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-Poly(A)

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET21b-Tipin-His

Plasmid

#23122

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Bacterial

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCYP16

Plasmid

#212849

Purpose

Expresses the Actinomadura pelletieri LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAFMW-AthHEN1

Plasmid

#104980

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKEK3228

Plasmid

#234090

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

aeBlue

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160-GCN4

Plasmid

#74608

Purpose

Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, GCN4, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Hook3(1-522)-SNAPf-Psc-StrepII

Plasmid

#111860

Purpose

C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-SNAPf (HOOK3 Human)

Tags

Psc, SNAPf, and Strep-II

Expression

Insect

Mutation

Optimised for expression in Sf9 cells

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-Fc-His

Plasmid

#72068

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Flag-Tipin

Plasmid

#23117

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only