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pGL2-deltaTAR-luciferase

Plasmid

#215896

Purpose

Expression of luciferase

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

luciferase

Use

Luciferase

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5417_p3WJdB_promoter dropout vector

Plasmid

#221191

Purpose

Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Use

Synthetic Biology

Promoter

Promoter Placeholder/Dropout Vector

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPSt

Plasmid

#203590

Purpose

Base plasmid for Plant STARR-seq terminator libraries.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Expression

Plant

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-yemiRFP670

Plasmid

#219856

Purpose

expression of Cyterm-yemiRFP670 in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-yemiRFP670

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 Cyterm-ymiRFP680

Plasmid

#219854

Purpose

expression of Cyterm-ymiRFP680 in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-ymiRFP680

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18-LucFF

Plasmid

#220496

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

Firefly Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.425

Plasmid

#203944

Purpose

Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.426

Plasmid

#203945

Purpose

Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.391

Plasmid

#203948

Purpose

Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

FnCas12a

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.385

Plasmid

#203936

Purpose

Up Flank. Neutral site. Up flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

desB UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.386

Plasmid

#203938

Purpose

Up Flank. Neutral site. Up flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

glgA1 UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.387

Plasmid

#203939

Purpose

Down Flank. Neutral site. Down flanking sequence of glycogen synthase A1 (glgA1, FEK30_14880) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

glgA1 DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.437

Plasmid

#203942

Purpose

Up Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

NS1 UP

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTΩSP0021

Plasmid

#187561

Purpose

Module for copper-inducible expression of firefly luciferase and nanoluc luciferase driven by minimal synthetic promoters with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTΩSP0022

Plasmid

#187562

Purpose

Module for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase and NanoLuciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-ymScarletI

Plasmid

#205512

Purpose

Expression of ymTq2-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymTq2-ymScarletl

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Ec-1 Triticum aestivum Metallothionein

Plasmid

#200296

Purpose

Bacterial expression plasmids for production of recombinant Triticum aestivum Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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