We narrowed to 12,459 results for: 109

1. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgMAPKAP1_2

   Plasmid

   #124911

   Purpose

   Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MAPKAP1 (MAPKAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgMAPKAP1_3

   Plasmid

   #124913

   Purpose

   Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MAPKAP1 (MAPKAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-RNF138-delta 86-105

   Plasmid

   #78922

   Purpose

   Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 86-105

   Promoter

   CMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-RNF138-delta 158-180

   Plasmid

   #78923

   Purpose

   Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 159-180

   Promoter

   CMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-RNF138-delta 189-215

   Plasmid

   #78924

   Purpose

   Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 189-215

   Promoter

   CMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. ORAI1-YFP T184M

   Plasmid

   #114181

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP T184M (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   T184M

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCGT-HCF-1-C600

   Plasmid

   #99364

   Purpose

   C-term of HCFC1 protein

   Depositor

   Winship Herr

   Article

   Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

   Insert

   HCF-1 C-term 1436-2035 (HCFC1 Human)
   

   Use

   Tags

   2x tandem T7 tag and C-myc

   Expression

   Mammalian

   Mutation

   Deletion of HCFC1 cDNA

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTTH+mEGFP-LANA-H1.4CTD

    Plasmid

    #157802

    Purpose

    Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA-H1.4CTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCardinal-Alpha-Actinin-19

    Plasmid

    #56152

    Purpose

    Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pFRT-TODestFLAGHA_RBPMS

    Plasmid

    #59388

    Purpose

    Destination vector with RBPMS for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23 T230A 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86017

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed threonine 230 to alanine

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86018

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed aspartic acid 637 to glycine

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pADSL-2XStrep

    Plasmid

    #125684

    Purpose

    expresses human ADSL protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    ADSL (ADSL Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Opto-GPR75

    Plasmid

    #106032

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR75 (GPR75 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

    Plasmid

    #14442

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

19. B52 + TIMELESS sgSTOP

    Plasmid

    #100713

    Purpose

    B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting TIMELESS (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. B52 + PARP4 sgSTOP

    Plasmid

    #100711

    Purpose

    B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting PARP4 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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