We narrowed to 15,323 results for: pET
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Plasmid#177076PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0120
Plasmid#177052PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Ovol1
Plasmid#172871PurposeMammalian expression vector with myc-tagged mouse Ovol1 coding sequenceDepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-E2
Plasmid#175243PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to YpetDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.13.hSyn.H2B.RFP
Plasmid#170372PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.4.EFS-NS.H2B-RFP
Plasmid#170365PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralPromoterEFS-NSAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.3.EFS-NS.H2B-RFP
Plasmid#170364PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralPromoterEFS-NSAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e1 sgRNA / hSpCas9
Plasmid#172830PurposeMammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e2 sgRNA / hSpCas9
Plasmid#172831PurposeMammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7-Qb model based
Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
Tags6x HISExpressionBacterialPromoterT7Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT139_GalL_nCDA1-VRERBE3
Plasmid#145102PurposeExpressing base editor nCDA1-VRERBE3 in yeast cellsDepositorInsertnCDA1-VRERBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT92_GalL_nCDA1Δ188-BE3
Plasmid#145052PurposeExpresses nCDA1Δ188-BE3 in yeast cellsDepositorInsertnCDA1Δ188-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-188AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT90_GalL_nCDA1Δ190-BE3
Plasmid#145050PurposeExpresses nCDA1Δ190-BE3 in yeast cellsDepositorInsertnCDA1Δ190-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-190AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT85_GalL_nCDA1Δ193-BE3
Plasmid#145048PurposeExpresses nCDA1Δ193-BE3 in yeast cellsDepositorInsertnCDA1Δ193-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-193AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT84_GalL_nCDA1Δ194-BE3
Plasmid#145047PurposeExpresses nCDA1Δ194-BE3 in yeast cellsDepositorInsertnCDA1Δ194-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-194AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT78_GalL_nCDA1Δ195-BE3
Plasmid#145044PurposeExpresses nCDA1Δ195-BE3 in yeast cellsDepositorInsertnCDA1Δ195-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-195AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph18
Plasmid#133029PurposeExpresses Xph18 in bacteriaDepositorInsertXph18
TagsHis10ExpressionBacterialMutationS63KPromoterT7Available SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph15
Plasmid#133030PurposeExpresses Xph15 in bacteriaDepositorInsertXph15
TagsHis10ExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 BICC FL
Plasmid#112998PurposeFor protein expression and purification of full-length Drosophila BicCDepositorInsertfull length BicC (BicC Fly)
ExpressionBacterialAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT
Plasmid#61796PurposeLuciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutatedDepositorInsertmouse Ywhab 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT
Plasmid#61793PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutatedDepositorInsertmouse Xbp1 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT
Plasmid#61791PurposeLuciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutatedDepositorInsertmouse Smad5 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT
Plasmid#61790PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutatedDepositorInsertmouse Map3k1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseMutationboth miR-200c MRE-2 and MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT
Plasmid#61786PurposeLuciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutatedDepositorInsertmouse Crtap 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT
Plasmid#61787PurposeLuciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutatedDepositorInsertmouse Fhod1 3'UTR with miR-200c MRE-2 mutated
UseLuciferaseMutationmiR-200c MRE-2 mutatedAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT
Plasmid#61788PurposeLuciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutatedDepositorInsertmouse Tob1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT
Plasmid#61794PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutatedDepositorInsertmouse Zfp36 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Snail1-3'UTR-MRE-1 MUT
Plasmid#61795PurposeLuciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutatedDepositorInsertmouse Snail1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT
Plasmid#61797PurposeLuciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutatedDepositorInsertmouse Mapk12 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I541P
Plasmid#61381PurposeExpresses Human full length RIPK1 with I541P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I541P mutation (RIPK1 Human)
TagsEGFP and HA tagExpressionMammalianMutationchanged I541 to PAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycMutationlacks E3 domain on N-term (del aa1-274)Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa31_2963
Plasmid#49688DepositorInsertECF31_2963
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only