We narrowed to 5,622 results for: crispr cas9 grna plasmid

1. gh119

   Plasmid

   #106789

   Purpose

   expression of gRNA targeting GCNT6

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GCNT6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. gh20

   Plasmid

   #106704

   Purpose

   expression of gRNA targeting GALNT20

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GALNT20
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. gh156

   Plasmid

   #106823

   Purpose

   expression of gRNA targeting GYLT1B (LARGE2)

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GYLT1B (LARGE2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. gh97

   Plasmid

   #106757

   Purpose

   expression of gRNA targeting POFUT2

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   POFUT2 (POFUT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR-cBEST-v2-PermE

   Plasmid

   #209446

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from ermE* promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_116_LVL2 cam

   Plasmid

   #179332

   Purpose

   NT-CRISPR plasmid for a single gRNA.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST_119_LVL2 cam

   Plasmid

   #179333

   Purpose

   NT-CRISPR plasmid for integration of multiple gRNAs.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TLCV2-LoxP

    Plasmid

    #127098

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system.

    Depositor

    Adam Karpf

    Insert

    Cas9-2A-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBC-MT1T2

    Plasmid

    #50593

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCBC-DT3T4

    Plasmid

    #50592

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCBC-MT3T4

    Plasmid

    #50595

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCBC-MT2T3

    Plasmid

    #50594

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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