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pT2/shCol1a1/GFP4_Seq1.3

Plasmid

#206136

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Publication

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-HRI-V5-IRES-BFP

Plasmid

#202435

Purpose

Lentiviral vector expression of HRI and TagBFP

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1alpha

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-shBIRC6-2

Plasmid

#202449

Purpose

DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 shRNA (BIRC6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgKCMF1

Plasmid

#202441

Purpose

Expression of a guide RNA targeting KCMF1

Depositor

William Hahn

Publication

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

KCMF1 gRNA (KCMF1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgCh2-2

Plasmid

#199637

Purpose

Tamoxifen-inducible expression of sgRNA control targeting intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/sh-Col1a1/GFP4_Seq1.2

Plasmid

#201403

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Publication

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pE-N1-RDH11

Plasmid

#161916

Purpose

Expresses untagged human RDH11. Confers G418 resistance.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Retinol dehydrogenase 11 (RDH11 Human)

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAB120

Plasmid

#167150

Purpose

MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

Depositor

Karen Sarkisyan

Publication

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

pNOS-nptII-ocsT

Expression

Plant

Promoter

pNOS

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJV132

Plasmid

#124254

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 3

Depositor

Stanley Riddell

Publication

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Promoter

MSCV

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6-5F5-mCherry

Plasmid

#73422

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

Depositor

Mattheos Koffas

Publication

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 5F5 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P5F5 (orthogonal T7-lac variant)

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBullet-vac-n

Plasmid

#53075

Purpose

destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Publication

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA TMEM 138-flag

Plasmid

#41628

Depositor

Joseph Gleeson

Publication

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
zgc:65890_L (OZ599)

Plasmid

#35239

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:65890 (zgc:65890 Zebrafish)

Use

Zebrafish targeting

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
unc119b_R (OZ582)

Plasmid

#35222

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting unc119b (unc119b Zebrafish)

Use

Zebrafish targeting

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
zgc:162148_R (OZ580)

Plasmid

#35220

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:162148 (micu1 Zebrafish)

Use

Zebrafish targeting

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
SDC3_L (OZ595)

Plasmid

#35235

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting SDC3

Use

Zebrafish targeting

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
col6a2_R (OZ594)

Plasmid

#35234

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting col6a2 (col6a2 Zebrafish)

Use

Zebrafish targeting

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
col6a2_L (OZ593)

Plasmid

#35233

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting col6a2 (col6a2 Zebrafish)

Use

Zebrafish targeting

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
flt1_L (OZ585)

Plasmid

#35225

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting flt1 (flt1 Zebrafish)

Use

Zebrafish targeting

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only