We narrowed to 24,434 results for: lars

1. pEPV-DsRed2-ER-5

   Plasmid

   #170571

   Purpose

   Expresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   EPV-DsRed2-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJUMP46-2A(sfGFP)

   Plasmid

   #126990

   Purpose

   Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p273 EF1a_mCherry2_hH11

   Plasmid

   #164852

   Purpose

   mCherry2-expression vector for human H11 locus

   Depositor

   Philip Jordan

   Article

   Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

   Insert

   mCherry2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYM29-BirA*

   Plasmid

   #160291

   Purpose

   Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1

   Depositor

   Martin Ott

   Article

   Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

   Insert

   BirA*
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-6xHis-GFP(+6)

   Plasmid

   #199160

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+6)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHCMV_VSBV_G

   Plasmid

   #207312

   Purpose

   Mammalian expression of Variegated Squirrel Bornavirus G protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSBV_G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHCMV_LUHF_GPC

   Plasmid

   #207290

   Purpose

   Mammalian expression of Lujo virus GPC protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   LUHF_GPC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHCMV_MACV_GPC

   Plasmid

   #207291

   Purpose

   Mammalian expression of Machupo virus GPC protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   MACV_GPC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT1002

   Plasmid

   #8621

   Depositor

   Robert Hartley

   Article

   Hartley et al Protein Peptide Letters 1996 3:225-231

   Insert

   barnase, barstar
   

   Use

   Tags

   as pMT416

   Expression

   Bacterial

   Mutation

   CI157 lambda repressor gene has two HindIII sites…

   Promoter

   Available since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pBAD/HisD-LSSmKate1

    Plasmid

    #31908

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSSmKate1
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-TTP(C147R)-GG-PUF

    Plasmid

    #52138

    Purpose

    Receiving vector for PUM-HD assembly and expression in mammalian cells

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Type

    Empty backbone

    Use

    Tags

    (GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD/HisD-LSSmKate2

    Plasmid

    #31909

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSSmKate2
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHCMV_ABLV_G

    Plasmid

    #207254

    Purpose

    Mammalian expression of Australian bat lyssavirus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    ABLV_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

    Plasmid

    #202689

    Purpose

    CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Inserts

    CopGFP
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pHCKAn-phoAp-GRFT

    Plasmid

    #158747

    Purpose

    low phosphate phoAp induction of Griffithsin

    Depositor

    Michael Lynch

    Article

    Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

    Insert

    phoAp Griffithsin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    phoA

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162233

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-GFP nanobody lag16lag2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-mito-ZifCY1

    Plasmid

    #36317

    Depositor

    Amy Palmer

    Article

    Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-9

    Insert

    mito-ZifCY1 genetically encoded Zn(II) sensor
    

    Use

    Tags

    see comment

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pSWAP_Entry_HAIB

    Plasmid

    #184869

    Purpose

    For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pcDNA3.1_27F3_hFc

    Plasmid

    #199619

    Purpose

    Encodes DARPin-hFc 27F3 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc 27F3
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSWAP_Entry_H2_beta_GM

    Plasmid

    #184871

    Purpose

    pSwap plasmid part with H2_beta homology arms and gentamycin cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pExp_CAG:EYFP

    Plasmid

    #51791

    Purpose

    Constitutively expresses yellow fluorescent protein , EYFP

    Depositor

    Ron Weiss

    Article

    Davidsohn et al ACS Synth Biol. 2014 Nov 17.

    Insert

    EYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJUMP27-1A(sfGFP)

    Plasmid

    #126974

    Purpose

    Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWS926

    Plasmid

    #123052

    Purpose

    LexA bacterial repressor

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexA-NLS [3a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLCRcala (p53)

    Plasmid

    #12136

    Depositor

    James Sherley

    Article

    Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pRK5-mWnt7b

    Plasmid

    #42283

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Wnt7b (Wnt7b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

28. 438-GST

    Plasmid

    #97092

    Purpose

    GST-tev-yORF

    Depositor

    Chris Jeans

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    GST tag and TEV cleavage site

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PCS2+ Cas9

    Plasmid

    #122948

    Purpose

    For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRK5-mWnt5a

    Plasmid

    #42279

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Wnt5a (Wnt5a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only