Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,363 results for: BLI

Showing: 4681 - 4700 of 9363 results

  1. pHH0103 CEP164 WW domain

    Plasmid
    #104276
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    CEP164 WW domain (CEP164 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104276-plasmid-map-sequence-id-203766

    Enlarge

  2. pHH0103 CEP164 WW domain

    Plasmid
    #104300
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    CEP164 WW domain (CEP164 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104300-plasmid-map-sequence-id-203483

    Enlarge

  3. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109062-plasmid-map-sequence-id-209541

    Enlarge

  4. hC73L-HA

    Plasmid
    #111527
    Purpose
    Expresses human KCNB1 C73L in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human C73L-Kv2.1-HA (KCNB1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C73L
    Promoter
    CMV
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111527-plasmid-map-sequence-id-211650

    Enlarge

  5. pHH0103 HECW2 WW domain #1

    Plasmid
    #104244
    Purpose
    Bacterial expression of WW domain #1 from HECW2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HECW2 WW domain #1 (HECW2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104244-plasmid-map-sequence-id-204378

    Enlarge

  6. His8:MBP-tev-Trp-eK-Flv

    Plasmid
    #83390
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83390-plasmid-map-sequence-id-175070

    Enlarge

  7. His8:MBP-tev-Asp-eK-Flv

    Plasmid
    #83389
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83389-plasmid-map-sequence-id-175068

    Enlarge

  8. His8:MBP-tev-His-eK-Flv

    Plasmid
    #83388
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83388-plasmid-map-sequence-id-175065

    Enlarge

  9. His8:MBP-tev-Lys-eK-Flv

    Plasmid
    #83387
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83387-plasmid-map-sequence-id-175064

    Enlarge

  10. His8:MBP-tev-Leu-eK-Flv

    Plasmid
    #83386
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83386-plasmid-map-sequence-id-175060

    Enlarge

  11. His8:MBP-tev-Arg-eK-Flv

    Plasmid
    #83385
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83385-plasmid-map-sequence-id-173975

    Enlarge

  12. His8:MBP-tev-Phe-eK-Flv

    Plasmid
    #83384
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83384-plasmid-map-sequence-id-173972

    Enlarge

  13. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid
    #87150
    Purpose
    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87150-plasmid-map-sequence-id-174939

    Enlarge

  14. pJM102: pHps2 RFP on pCM66T

    Plasmid
    #74741
    Purpose
    pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    pHps2 (extracted from Methylovorus glucosetrophus…
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74741-plasmid-map-sequence-id-141458

    Enlarge

  15. pJK103

    Plasmid
    #73265
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73265-plasmid-map-sequence-id-134809

    Enlarge

  16. pJK610

    Plasmid
    #72531
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72531-plasmid-map-sequence-id-132619

    Enlarge

  17. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

    Enlarge

  18. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

    Enlarge

  19. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

    Enlarge

  20. pJK586

    Plasmid
    #72459
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72459-plasmid-map-sequence-id-133898

    Enlarge

Showing: 4681 - 4700 of 9363 results