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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway Cloning

Mutation

delPYD (I125M-END)

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway Cloning

Mutation

R262W

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway Cloning

Mutation

delPYD (I125M-END)

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-gI-BlastR

Plasmid

#207179

Purpose

Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFVp

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SV40p-gI-BlastR

Plasmid

#207182

Purpose

Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gATRIP_A

Plasmid

#211537

Purpose

sgRNA-A against ATRIP

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ATRIP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaD

Plasmid

#210448

Purpose

Express waaD under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaD

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaJ

Plasmid

#210450

Purpose

Express waaJ under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaJ

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaK

Plasmid

#210451

Purpose

Express waaK under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaK

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaX

Plasmid

#210459

Purpose

Express waaX under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaX

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC6L gRNA2

Plasmid

#211633

Purpose

sgRNA-2 agsinst ERCC6L

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC6L

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ZWILCH gRNA2

Plasmid

#211635

Purpose

sgRNA-2 against ZWILCH

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ZWILCH

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ZWILCH gRNA1

Plasmid

#211634

Purpose

sgRNA-1 against ZWILCH

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ZWILCH

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC6L gRNA1

Plasmid

#211632

Purpose

sgRNA-1 against ERCC6L

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC6L

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC1 gRNA2

Plasmid

#211631

Purpose

sgRNA-2 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-sp6-kcnk4b

Plasmid

#213123

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk4b (kcnk4b Zebrafish)

Use

Cloning

Mutation

Stop codon removed

Promoter

T7

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk19/kcnk12l

Plasmid

#209001

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk19 (kcnk12l Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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