We narrowed to 5,570 results for: SPI

1. 3020_pETcon-SARS-CoV-2-RBD_N501Y

   Plasmid

   #184405

   Purpose

   yeast surface display of the SARS-CoV-2 Alpha variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   N501Y

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-α-COPI-WD40-Arg57Ala

   Plasmid

   #180759

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   α-COPI-WD40-Arg57Ala
   

   Use

   Tags

   C-terminal strep tag

   Expression

   Mammalian

   Mutation

   Encodes residues 1-327; mutations: Arg57Ala, Leu1…

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-α-COPI-WD40-Tyr139Ala

   Plasmid

   #180761

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   α-COPI-WD40-Tyr139Ala
   

   Use

   Tags

   C-terminal strep tag

   Expression

   Mammalian

   Mutation

   Encodes residues 1-327; mutations: Tyr139Ala, Leu…

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-α-COPI-WD40-Asp115Ala

   Plasmid

   #180760

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   α-COPI-WD40-Asp115Ala
   

   Use

   Tags

   C-terminal strep tag

   Expression

   Mammalian

   Mutation

   Encodes residues 1-327; mutations: Asp115Ala, Leu…

   Promoter

   CMV

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6.2-coHPDL H163A H258A 3xFLAG-V5

   Plasmid

   #174131

   Purpose

   Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized, H163A, H258A

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.2-coHPDL H258A 3xFlag-V5

   Plasmid

   #174130

   Purpose

   Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Codon optimized, H258A

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX022

   Plasmid

   #167148

   Purpose

   MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   C-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX021

   Plasmid

   #167147

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   N-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ282 (TsCas12a)

   Plasmid

   #138114

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   TsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR3-vsv-INCENP d543-746

    Plasmid

    #108473

    Purpose

    expression of vsv-INCENP d543-746

    Depositor

    Susanne Lens

    Article

    Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

    Insert

    INCENP d543-746 AA (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Delta Alpha helix, aa543-746 deleted

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1 hSlp4-a I18A

    Plasmid

    #40043

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a I18A (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Isoleucine 18 to Alanine

    Promoter

    CMV promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-C1 hSlp4-a linker

    Plasmid

    #40040

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a linker (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    linker domain (144-353)

    Promoter

    cmv

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

13. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-EGFP-Tau P301L

    Plasmid

    #46908

    Purpose

    expresses EGFP tagged Tau P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-p300

    Plasmid

    #191764

    Purpose

    Expression of EGFP fused to core p300 histone acetyltransferase

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    FRB-EGFP-p300 core (EP300 Human)
    

    Use

    Tags

    EGFP (N-terminal of p300 core) and FRB (N-termina…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFPC1-hVAP-B

    Plasmid

    #104448

    Purpose

    Expression of human VAP-B fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-myc-ACE2

    Plasmid

    #141185

    Purpose

    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EB3-mNeonGreen

    Plasmid

    #191761

    Purpose

    Expression of mNeonGreen fused to EB3

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    EB3-mNeonGreen (MAPRE3 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

    Plasmid

    #133785

    Purpose

    Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    PSD95-EGFP (Dlg4 Rat)
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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