We narrowed to 8,304 results for: construct

1. 1D3 HC

   Plasmid

   #170670

   Purpose

   Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

   Depositor

   Ronald Jemmerson, Rafel Radi

   Article

   Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

   Insert

   HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 1D3 LC

   Plasmid

   #170669

   Purpose

   Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

   Depositor

   Ronald Jemmerson, Rafel Radi

   Article

   Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

   Insert

   LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. lentiCRISPRv2_PROX1_1+3

   Plasmid

   #121176

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDUAL CLDN (GFP)

   Plasmid

   #86981

   Purpose

   Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   CLDN1 (CLDN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSegfpcherry

   Plasmid

   #22465

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   EGFP-mCherry
   

   Use

   Injection construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

   Plasmid

   #86456

   Purpose

   Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TALEN_C63

   Plasmid

   #40788

   Purpose

   Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI

   Depositor

   Gang Bao

   Insert

   Transcription Activator Like Effector Nuclease
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   +63 C Terminus, Codon Optimized FokI Endonuclease

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKlf4_Halo_Ty2_BactBsd

   Plasmid

   #235512

   Purpose

   C-term HALO targeting construct

   Depositor

   Brian Hendrich

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Mouse Targeting

   Tags

   HALO, 2xTy

   Expression

   Mutation

   None

   Promoter

   beta actin

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid

    #160631

    Purpose

    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.

    Depositor

    Diego Orzaez

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U626

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCASCADE-gltA2-udhA

    Plasmid

    #65819

    Purpose

    gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA2-udhA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALEN_C18

    Plasmid

    #47974

    Purpose

    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Shortened C-Terminus,Codon Optimized FokI Endonuc…

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pInt_attP1_LCS_kanR

    Plasmid

    #205300

    Purpose

    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid

    #135391

    Purpose

    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

    Depositor

    Fan Wang

    Insert

    TeLC-P2A-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AARS_C2_pNIC-Bio3

    Plasmid

    #153047

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid

    #200661

    Purpose

    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AARS_C1_pNIC-Bio3

    Plasmid

    #153046

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid

    #200660

    Purpose

    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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