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PCx35CT-S276A, S298ter

Plasmid

#42914

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser276 to Ala and Ser298 stop codon

Promoter

T7

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S110A, S276A

Plasmid

#42926

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser110 to Ala and Ser276 to Ala

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S110A, S276A, S298ter

Plasmid

#42927

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser110 to Ala, Ser276 to Ala, and Ser298 to stop …

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pNH066

Plasmid

#42162

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP Tag-RT-::mTFP1

Tags

TAP-TAg

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH009

Plasmid

#42146

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP-tag::mTFP1

Tags

TAP-Tag

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-THAP11(AA132-313)

Plasmid

#37002

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11(AA132-313) (THAP11 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 132 to 313

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pBacPAK8-VHL(1-155)

Plasmid

#29500

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Expression

Insect

Mutation

VHL(1-155)only

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK RHEBL1 3'UTR

Plasmid

#19858

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

RHEBL1 3'UTR (RHEBL1 Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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BacPAK Flag-NFRKB 1-101

Plasmid

#19792

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

Flag and His

Expression

Insect

Mutation

only contains amino acids 1-101

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pET19b NFRKB 1-101

Plasmid

#19787

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

His

Expression

Bacterial

Mutation

only contains amino acids 1-101

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244

Plasmid

#17859

Depositor

David McKay

Article

Kwon et al J Mol Biol. 2003 Jul 4. 330(2):185-95.

Insert

Haemophilus influenzae heat shock loci U and V

Expression

Bacterial

Mutation

coding sequence for residues 108-243 of HslU (the…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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3205 pCRII Mcl-1 fragment

Plasmid

#8757

Depositor

Stanley Korsmeyer

Article

Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

Insert

Mcl-1 cDNA PCR fragment (Mcl1 Mouse)

Expression

Bacterial

Mutation

Base pairs 943-1223.

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pFSW Syt1-C2B R398,399Q IRES GFP

Plasmid

#133819

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

R398,399Q

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B K326,327E IRES GFP

Plasmid

#133820

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K326,327E

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pHWBF06EC

Plasmid

#130827

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Availability

Academic Institutions and Nonprofits only

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SUN2delCC

Plasmid

#130836

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of coiled coil domain

Promoter

CaMV 35S

Availability

Academic Institutions and Nonprofits only

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SUN2delSUNec

Plasmid

#130847

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Mutation

Deletion of SUN domain

Availability

Academic Institutions and Nonprofits only

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SUN2delCCec

Plasmid

#130848

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Mutation

Deletion of coiled coil domain

Availability

Academic Institutions and Nonprofits only

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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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U6GRNA

Plasmid

#68370

Purpose

gRNA scaffold with hU6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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