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pCAG-Flpe:GFP
Plasmid#13788PurposeMammalian expression of enhanced form of Flp recombinase fused to GFPDepositorInsertFlpe-GFP fusion protein
UseFlp/frtTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV
Plasmid#16403PurposeShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Fab_8D3_2_H-H6
Plasmid#176075PurposeExpress His6 tagged heavy chain of Fab_8D3_2 in mammalian cellsDepositorInsertHis6 tagged heavy chain of Fab_8D3_2
UseTagsHis tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Fab_8D3_2_L
Plasmid#176076PurposeExpress light chain of Fab_8D3_2 in mammalian cellsDepositorInsertLight chain of Fab_8D3_2
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP
Plasmid#181906PurposeFluorescently tagged dead Cas9DepositorInsertdCas9
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mGreenLantern:ALFA
Plasmid#219644PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest methodDepositorInsertmGreenLantern:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDG461
Plasmid#100902PurposeSpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-EGFPExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
