We narrowed to 12,459 results for: 109

1. pLVTHM-mBrn2-3xflag

   Plasmid

   #206390

   Purpose

   Expresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7-δ

   Plasmid

   #197259

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-3 δ (AP3D1 Human)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Val 549 Ile substitution; silent substitutions in…

   Promoter

   ADH1

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBridge-tyrosinase.σ3A

   Plasmid

   #197513

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-3 σ3A
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   silent substitution in codon 2 of tyrosinase tail…

   Promoter

   ADH1 and MET25

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIneo-lambdaN-HA-HsDCP1a-Y36A_P

   Plasmid

   #147218

   Purpose

   Mammalian Expression of HsDCP1a-Y36A

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsDCP1a-Y36A (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and G1716T compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-EGFP-C1-HsDCP1a-Y36A_P

   Plasmid

   #147221

   Purpose

   Mammalian Expression of HsDCP1a-Y36A

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsDCP1a-Y36A (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and T1716G compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM41P-HsUPF3b-iso2_145-470_K

   Plasmid

   #146754

   Purpose

   Bacterial Expression of HsUPF3biso2_145-470

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsUPF3biso2_145-470 (UPF3B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1-HsUPF3b-iso2_145-470_K

   Plasmid

   #146759

   Purpose

   Bacterial Expression of HsUPF3biso2_145-470

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsUPF3biso2_145-470 (UPF3B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLNHA-C1-HsUPF3biso2_I

   Plasmid

   #146568

   Purpose

   Mammalian Expression of HsUPF3Biso2

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsUPF3Biso2 (UPF3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation T510C compared to the sequenc…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGADT7-ε

   Plasmid

   #197261

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-4 ε (AP4E1 Human)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitutions in codons 905, 1129 and 1137

   Promoter

   ADH1

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-Duet1-6xHis-TEV-NDP52

    Plasmid

    #190194

    Purpose

    Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATX3-JD

    Plasmid

    #184247

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    Ataxin-3 Josephin domain (ATXN3 Human)
    

    Use

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Premature stop codon V183X

    Promoter

    T7 promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PHR-GFP-VP16

    Plasmid

    #183927

    Purpose

    Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

    Plasmid

    #178825

    Purpose

    Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    QuasAr6b_citrine
    

    Use

    Cre/Lox and Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CBR3_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176961

    Purpose

    Mammalian expression vector encoding CBR3 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CBR3 (CBR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

    Plasmid

    #105196

    Purpose

    targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

    Depositor

    Peter Mombaerts

    Article

    Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

    Insert

    Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

16. p3xFLAG-CMV-Drosha(W1258)

    Plasmid

    #171535

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    a Drosha stopping at W1258

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xFLAG-CMV-Drosha (P426)

    Plasmid

    #171537

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    a Drosha mutant starting from P426

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFLAG-CMV-Drosha (E419)

    Plasmid

    #171534

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    a Drosha mutant stopping at E419

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK-Drosha-FLAG(delta6)

    Plasmid

    #171532

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    small deletions of Drosha_1271A to 1276G_

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b p97-Strep-His E314Amb

    Plasmid

    #169021

    Purpose

    Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His and SBP

    Expression

    Bacterial

    Mutation

    E314TAG

    Promoter

    T7

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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