We narrowed to 9,638 results for: Ada;

1. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-spCas9 ABE-Umax-ex1-rest1

   Plasmid

   #222140

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. opt.a_pSTC0-zeo

   Plasmid

   #202347

   Purpose

   Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   opt.a
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. opt.b_pSTC0-zeo

   Plasmid

   #202348

   Purpose

   Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   opt.b
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. neg.ctrl_pSTC0-zeo

   Plasmid

   #202350

   Purpose

   Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

   Depositor

   Nicholas Gauthier

   Article

   Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

   Insert

   neg. ctrl
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Q54A

   Plasmid

   #217912

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP Q54A
   

   Use

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Glutamine at position 55 and 267 mutated to Alani…

   Promoter

   T5

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. T40A

   Plasmid

   #217911

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP T40A
   

   Use

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Threonine at position 41 and 253 mutated to Alani…

   Promoter

   T5

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJM166B

   Plasmid

   #218123

   Purpose

   For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   FRB (MTOR Human)
   

   Use

   Tags

   rpoZ

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-GCaMP6f-WPRE

   Plasmid

   #216275

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human Synapsin 1 (hSyn)

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

    Plasmid

    #208869

    Purpose

    For mammalian expression of NAP1 (delta-TBK1)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T1-GST-TEV-GFP-NAP1

    Plasmid

    #208870

    Purpose

    For expression in E. coli of GFP-NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GFP and GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL6-H2B-iRFP720-bla

    Plasmid

    #189991

    Purpose

    Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Human histone H2B
    

    Use

    Lentiviral

    Tags

    iRFP720

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1-Cdc45

    Plasmid

    #208796

    Purpose

    Xenopus laevis FLAG5-His10-TEV-Cdc45

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    Cdc45 (cdc45.L Frog)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-DONSON-WM

    Plasmid

    #208798

    Purpose

    Xenopus laevis His6-Myc-DONSON (W234L, M463T)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    W234L; M463T

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-DONSON-S437A

    Plasmid

    #208803

    Purpose

    Xenopus laevis His6-Myc-DONSON (S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    S437A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-DONSON-ΔD1

    Plasmid

    #208804

    Purpose

    Xenopus laevis His6-DONSON (1-155aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    ΔD1, deletion of 1-155aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNKX2-5-GFP-T2A

    Plasmid

    #203608

    Purpose

    Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

    Depositor

    Matthew Birket

    Article

    Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

    Insert

    NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa.HALO-KIF3A tail 419-702

    Plasmid

    #193924

    Purpose

    Mammalian expression of Halo-tagged Kif 3A tail 419-702

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3A (KIF3A Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMx/ATP10A(E203Q)-HA

    Plasmid

    #209258

    Purpose

    Mammalian expression of ATP10A mutant E203Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10A (ATP10A Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    E203Q

    Promoter

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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