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SpVasa-P662K-GFP

Plasmid

#185591

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

P662K

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-S83K-GFP

Plasmid

#185588

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S83K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

S83K

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-E441Q-GFP

Plasmid

#185590

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-E441Q

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

E441Q

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-mCherry-LOV

Plasmid

#185585

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry-LOV

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic_D

Plasmid

#146143

Purpose

Mammalian Expression of tubgenomic

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-K332N-GFP

Plasmid

#185592

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-K332N

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

K332N

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-S636K-GFP

Plasmid

#185589

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S636K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

S636K

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPatL1_70-770_J

Plasmid

#146662

Purpose

Mammalian Expression of HsPatL1_70-770

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770 (PATL1 Human)

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-NTerminus8xHis-T500

Plasmid

#178421

Purpose

Empty backbone. T7Max promoter with N-His Tag

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

8xHis

Expression

Bacterial

Promoter

T7Max

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-173a-16X

Plasmid

#165453

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SZ1-GST-mCherry-RGG

Plasmid

#176646

Purpose

SynZip1 linked surfactant protein that localizes to RGG condensate interface

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

SZ1-GST-mCherry-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1193

Plasmid

#167614

Purpose

Express TonB H20N

Depositor

Kathleen Postle

Article

Swayne et al J Bacteriol. 2011 Aug;193(15):3693-701.

Insert

tonB H20N

Expression

Bacterial

Mutation

tonB with H20N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1064

Plasmid

#167607

Purpose

Express ExbD D25N protein

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbD D25N

Expression

Bacterial

Mutation

exbD with D25N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP381

Plasmid

#167592

Purpose

Express TonB H20A protein

Depositor

Kathleen Postle

Article

Larsen et al J Bacteriol. 2007 Apr;189(7):2825-33. do

Insert

tonB H20A

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 TagRFP CTAG

Plasmid

#153401

Purpose

Red fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

TagRFP CTAG

Expression

Plant

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-171a-16X

Plasmid

#165452

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-nonpalindromic-169an-4X

Plasmid

#165413

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-167a-16X

Plasmid

#164494

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-169a-16X

Plasmid

#164495

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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