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pST_DHN_OsLEA5 (pBS0793)

Plasmid

#185259

Purpose

For the mammalian expression of the rice plant protein DHN_OsLEA5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_OsLEA5

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdHsAPO-44 (pBS0746)

Plasmid

#185237

Purpose

For the mammalian expression of the Chinese giant salamander and human fusion protein AdHsAPO-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdHsAPO-44

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_52-358 (pBS0744)

Plasmid

#185235

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_52-358

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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R9-FS1

Plasmid

#125208

Purpose

Expressing human Rad9b frameshift mutation found in a neural tube defect patient

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Mutation

p.S318SfsX28

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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R9-FS2

Plasmid

#125209

Purpose

Expressing human Rad9b frameshift mutation found in a neural tube defect patient

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Mutation

p.A401Rfs4X

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDK366

Plasmid

#134196

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

5xRD SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

5xRD, Alternate Start, G5 hardeneded

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph20-Stg

Plasmid

#133035

Purpose

Expresses Xph20-Stg in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-Stg

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph18-ETWV

Plasmid

#133036

Purpose

Expresses Xph18-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph18-Stg

Plasmid

#133037

Purpose

Expresses Xph18-Stg in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18-Stg

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph15-ETWV

Plasmid

#133038

Purpose

Expresses Xph15-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph0-ETWV

Plasmid

#133039

Purpose

Expresses Xph0-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph0-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pIG-TAT-Xph20-ETWV

Plasmid

#133040

Purpose

Expresses TAT-Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

His8, TAT, and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-M4

Plasmid

#114036

Purpose

Expression of HEC1 M4 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M4 (NDC80 Human)

Expression

Mammalian

Mutation

M4 (K146E, R153E, K156E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-M2

Plasmid

#114035

Purpose

Expression of HEC1 M2 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M2 (NDC80 Human)

Expression

Mammalian

Mutation

M2 (K81E, N87E, K89E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16 (B695)

Plasmid

#92031

Purpose

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16AD

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse Nanog locus 3' probe

Plasmid

#60037

Purpose

Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

Nanog (Nanog Mouse)

Use

Unspecified; Cloning

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-Delta-ATG1-mMCL1

Plasmid

#45823

Purpose

This plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

Delta ATG1-mMCL1

Expression

Mammalian

Mutation

The first amino acid (ATG) is deleted from the fu…

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a I18A

Plasmid

#40066

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Isoleucine 18 to Alanine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a W118S

Plasmid

#40065

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a W118S (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Tryptophane 118 to Serine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only