We narrowed to 7,165 results for: trac

1. pZH520

   Plasmid

   #102673

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNAtet-phaZ

   Plasmid

   #169874

   Purpose

   Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   sgRNA towards phaZ in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZH521

   Plasmid

   #102674

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCOLA kiwi sensor sfGFP

   Plasmid

   #107368

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   kiwi-detecting toehold + sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFP.R275

   Plasmid

   #138224

   Purpose

   Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZH517

   Plasmid

   #102670

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLMB757

   Plasmid

   #83792

   Purpose

   Fructose biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   Region upstream RL0489 (frcB) containing all of RL0488 (frcR)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFP.R371

   Plasmid

   #138241

   Purpose

   Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKF-P14MM2AG5h

   Plasmid

   #128254

   Purpose

   Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rap-MESA-20-M-Gal4

    Plasmid

    #84554

    Purpose

    MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    Rap-MESA-FRB-20-M-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MXS_PGK::rtTA3-bGHpA

    Plasmid

    #62446

    Purpose

    MXS_chaining vector with PGK::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZH519

    Plasmid

    #102672

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZH522

    Plasmid

    #102675

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLMB713

    Plasmid

    #83785

    Purpose

    Salicylic acid biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL1329 (salA) containing all of RL1328 (salR)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-mEGFP-PKCg-C1

    Plasmid

    #112266

    Purpose

    ITRACKgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW002-PpsbA

    Plasmid

    #108236

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZH518

    Plasmid

    #102671

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFP.R400

    Plasmid

    #138175

    Purpose

    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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