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CS-TRE-BclXL-Ubc-rtTA

Plasmid

#198189

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Article

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hBCLXL (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-hGDNF

Plasmid

#122035

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

hGDNF (GDNF Human)

Use

AAV

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

Plasmid

#113163

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Kimberley Evason, Didier Stainier

Article

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Promoter

cryaa (crystallin) and fabp10a

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC763 - pX458 with rat Rosa26 gRNA A

Plasmid

#113161

Purpose

A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat Rosa26

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

Plasmid

#179465

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninA-

Plasmid

#229698

Purpose

Transient expression of GFP-alpha-cateninA- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L785A, I792A, V796A

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

Plasmid

#112683

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-iCre and Nuclear localization signal

Promoter

CMV-IE

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

Plasmid

#112719

Purpose

An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

AAV

Mutation

D10A

Promoter

Mecp2

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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