We narrowed to 11,977 results for: pcDNA

1. pCDNA3 Flag Jip1 JBD

   Plasmid

   #15751

   Purpose

   binds to Jnk

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted 1-126 and 282-660

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)-ExRai-AKAR-Kras

   Plasmid

   #118408

   Purpose

   Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-Kras
   

   Use

   Tags

   C-terminal targeting sequence from Kras

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-S586A-VLCAD-FLAG

   Plasmid

   #55824

   Purpose

   Expresses S586A VLCAD mutant in mammalian cells

   Depositor

   Yoshimi Homma

   Article

   Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

   Insert

   Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   changed Serine 586 toAlanine

   Promoter

   CMV

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-GFP-2AFP-KDEL

   Plasmid

   #154349

   Purpose

   Mammalian expression of 2AFP

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   2AFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-FRB-LplA(W37V)

   Plasmid

   #35608

   Depositor

   Alice Ting

   Article

   Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

   Insert

   FRB-LplA(W37V)
   

   Use

   Tags

   FLAG (internal fusion), HA, and His Tag

   Expression

   Mammalian

   Mutation

   LplA has Trp37 to Val mutation

   Promoter

   CMV

   Available since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194684

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3 5' cMyc- SMCI-S966A

   Plasmid

   #32371

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   S996A

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-myc-p110α-CAAX

   Plasmid

   #39490

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

   Insert

   p110 (PIK3CA Bovine)
   

   Use

   Tags

   CAAX and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-TAT-1-delta2-26-FLAG

   Plasmid

   #35983

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1 aa 2-26 deleted

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Lyn-sACt-mCherry

    Plasmid

    #138216

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    Lyn-sACt-mCherry (Adcy10 Rat)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid

    #136454

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO GFP DNAJA4

    Plasmid

    #19494

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA4 (DNAJA4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Flag-YOD1 C160S

    Plasmid

    #49785

    Purpose

    Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    Flag-tag

    Expression

    Mutation

    C160S

    Promoter

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA-zeo-hFJX1-myc

    Plasmid

    #46930

    Purpose

    expression of human FJX1-myc in mammalian cells

    Depositor

    R.D. Beauchamp, Natasha Deane

    Insert

    FJX1 (FJX1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.2/V5 hsa-mir-214

    Plasmid

    #26313

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-214 (MIR214 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

18. GeNL(Ca2+)_250/pcDNA3

    Plasmid

    #85206

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_250
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-NBid-mMaple-CBid

    Plasmid

    #164049

    Purpose

    Optogenetic manipulation of cell apoptosis negative control

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-mMaple-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

    Plasmid

    #21800

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Use

    Tags

    GST and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge