We narrowed to 5,886 results for: arl

1. ph7SK-gRNA

   Plasmid

   #53189

   Purpose

   Expresses the S. pyogenes sgRNA from the 7SK promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human 7SK

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSKI

   Plasmid

   #232484

   Purpose

   pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

   Depositor

   Carlos Silva García

   Article

   Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

   Insert

   pSKI
   

   Expression

   Worm

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor MCS Rosa26

   Plasmid

   #37200

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Inserts

   Rosa26 left targeting arm
   MCS
   Rosa26 right targeting arm
   

   Use

   Genomic targeting - zinc finger

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

   Plasmid

   #20298

   Purpose

   Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   channelrhodopsin-2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   humanized ChR2 gene with histidine 134 changed to…

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

   Plasmid

   #174665

   Purpose

   Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

   Depositor

   Eli Bar

   Inserts

   LUC2
   mCherry:T2A:Hygro
   

   Use

   Lentiviral and Luciferase

   Tags

   mCherry and hygromycin dual reporter gene linked …

   Expression

   Mammalian

   Promoter

   Human cytomegalovirus immediate early enhancer/pr…

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2

   Plasmid

   #60625

   Purpose

   Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 mouse MyoD
   

   Use

   Lentiviral

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-DEST47-SIRT7

   Plasmid

   #242137

   Purpose

   Expression in mammalian cells

   Depositor

   J. Carl Barrett, Izumi Horikawa

   Article

   Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

   Insert

   SIRT7 (SIRT7 Human)
   

   Tags

   Cycle 3 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p102_gRNA_Sa_mClover

   Plasmid

   #213778

   Purpose

   Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sa gRNA
   

   Use

   CRISPR and Lentiviral

   Promoter

   mU6

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RUBYm2

   Plasmid

   #232175

   Purpose

   Introduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression

   Depositor

   Yiping Qi

   Article

   Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

   Insert

   RUBY mutant2 and CaMV 35S promoter
   

   Expression

   Plant

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hNox5

    Plasmid

    #69354

    Purpose

    expresses human Nox5 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

    Insert

    hNox5 (NOX5 Human)
    

    Tags

    no

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEVY-U

    Plasmid

    #51230

    Purpose

    bi-directional expression vector for yeast, constitutively active, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GPD/ADH1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/VChR1-EYFP

    Plasmid

    #20955

    Purpose

    Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

    Insert

    volvox channelrhodopsin-1
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEED-mGreenLantern:ALFA

    Plasmid

    #219644

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern:ALFA SEED Cassette
    

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pComb3HTT

    Plasmid

    #63900

    Purpose

    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2

    Plasmid

    #60627

    Purpose

    Expresses p65 mouse MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    p65 mouse MyoD
    

    Use

    Lentiviral

    Promoter

    TRE

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-Rncp-iGluSnFR1

    Plasmid

    #107336

    Purpose

    Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    Rncp-iGluSnFR1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid

    #105714

    Purpose

    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

    Depositor

    Rylan Larsen

    Has Service

    AAV8

    Insert

    GCaMP6s
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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