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Plasmid#238038PurposeEncodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterlacAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-BFP
Plasmid#227505PurposeBackbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistenceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVRha
Plasmid#229553PurposeBroad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ΔndhD2.km
Plasmid#185532Purposea pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locusDepositorInsertnptII gene flanked by ndhD2 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB53
Plasmid#226309PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-3
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA324
Plasmid#214741PurposeLow copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV015
Plasmid#214738PurposeLow copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-mRFP
Plasmid#170982PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS193
Plasmid#197143PurposescCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS138
Plasmid#197142PurposescCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-S11-GFP1–9
Plasmid#125666PurposeBinary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertER-S11-GFP1–9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-AM
Plasmid#153427PurposeRetroviral (MSCV) expression of ametrine fluorescent proteinDepositorInsertametrine
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-LO
Plasmid#153428PurposeRetroviral (MSCV) expression of LSSmOrange fluorescent proteinDepositorInsertLSSmOrange
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8_005
Plasmid#123885PurposeEncodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK systemDepositorInsertF1-KanR-ColE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_38J
Plasmid#91154PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt IIDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB434
Plasmid#68699PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB433
Plasmid#68698PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB410
Plasmid#68675PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB413
Plasmid#68678PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB233
Plasmid#68624PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq20-T38S38-0X
Plasmid#49522PurposeKanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertconstitutive TetR38
UseTagsExpressionBacterialMutationPromoterPtb38Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAB18-GFP
Plasmid#106984PurposeABA signaling reporter, GFP under the RAB18 promoterDepositorInsertsGFP
UseTagsExpressionPlantMutationPromoterRAB18Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mFlagEts1
Plasmid#86099Purposeexpresses flag tagged Ets1 in mammalian cellsDepositorInsertEts1 (Ets1 Mouse)
UseTagsFlag tag; kanamycin selectionExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLX_307 GFP
Plasmid#184492PurposeLentivirus expression for GFPDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-p53D278N
Plasmid#22727DepositorInsertTrp53 (Trp53 Mouse)
UseRetroviralTagsExpressionMammalianMutationD278NPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only