Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 17,982 results for: gateway

Showing: 1151 - 1200 of 17982 results

  2. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid
    #224497
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    224497-plasmid-map-sequence-id-447355

    Enlarge

  3. p2R3a-VenusYFP-3AT

    Plasmid
    #71269
    Purpose
    Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    VenusYFP
    Use
    Gateway
    Tags
    4xGly linker and T3A pea Pisum sativum ribulose-1…
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71269-plasmid-map-sequence-id-129809

    Enlarge

  4. p2R3a-3xVenusYFP-OcsT

    Plasmid
    #71271
    Purpose
    Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    3 times VenusYFP
    Use
    Gateway
    Tags
    octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71271-plasmid-map-sequence-id-129818

    Enlarge

  5. p2R3a-GUS-OcsT

    Plasmid
    #71267
    Purpose
    Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Beta-glucuronidase
    Use
    Gateway
    Tags
    2xGly linker and octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71267-plasmid-map-sequence-id-129805

    Enlarge

  6. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

    Enlarge

  7. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid
    #224496
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224496-plasmid-map-sequence-id-447065

    Enlarge

  8. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

    Plasmid
    #224512
    Purpose
    A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    3xHA-BirA-T2A-mCherry-Caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224512-plasmid-map-sequence-id-446939

    Enlarge

  10. pGP8G-ccdB

    Plasmid
    #83884
    Purpose
    Destination vector for integration at HIS3, expression from the GPD promoter
    Depositor
    Jennifer Nemhauser
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83884-plasmid-map-sequence-id-160280

    Enlarge

  11. Hs.NRAS

    Plasmid
    #83173
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83173-plasmid-map-sequence-id-158136

    Enlarge

  12. pDONR221-TMPRSS11D

    Plasmid
    #158447
    Purpose
    Gateway Cloning compatible entry vector for the human TMPRSS11D gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158447-plasmid-map-sequence-id-308252

    Enlarge

  13. pCAG/HA-hBIG2

    Plasmid
    #226254
    Purpose
    Expresses N-terminally HA-tagged hBIG2 in mammalian cells
    Depositor
    Hye-Won Shin
    Insert
    BIG2 (ARFGEF2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226254-plasmid-map-sequence-id-454238

    Enlarge

  14. Human-Dysferlin-v1

    Plasmid
    #60217
    Purpose
    Human dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60217-plasmid-map-sequence-id-96331

    Enlarge

  15. pCAG/HA-hBIG1

    Plasmid
    #226253
    Purpose
    Expresses N-terminally HA-tagged hBIG1 in mammalian cells
    Depositor
    Hye-Won Shin
    Insert
    BIG1 (ARFGEF1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226253-plasmid-map-sequence-id-454236

    Enlarge

  17. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid
    #135241
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135241-plasmid-map-sequence-id-268090

    Enlarge

  18. p663-UBC-TurboID-V5-dsRed_IDG-K

    Plasmid
    #135261
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135261-plasmid-map-sequence-id-268068

    Enlarge

  19. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

    Enlarge

  20. p663-UBC-miniTurbo-V5-eGFP_IDG-K

    Plasmid
    #135263
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135263-plasmid-map-sequence-id-268067

    Enlarge

  21. pDONR221-TMPRSS2

    Plasmid
    #158441
    Purpose
    Gateway Cloning compatible entry vector for the human TMPRSS2 gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158441-plasmid-map-sequence-id-308256

    Enlarge

  22. Mouse-Dysferlin-Transcript-Variant1

    Plasmid
    #60214
    Purpose
    Mouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (Dysf Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60214-plasmid-map-sequence-id-96316

    Enlarge

  23. R777-E005 Hs.AKT3

    Plasmid
    #70289
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    70289-plasmid-map-sequence-id-125738

    Enlarge

  24. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid
    #135240
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135240-plasmid-map-sequence-id-268088

    Enlarge

  25. p667-UBC-hcRed-V5-miniTurbo_IDG-K

    Plasmid
    #135237
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135237-plasmid-map-sequence-id-311572

    Enlarge

  26. R777-E085 Hs.FGFR2

    Plasmid
    #70369
    Purpose
    Gateway ORF clone of human FGFR2 [NM_000141.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR2 (FGFR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70369-plasmid-map-sequence-id-125818

    Enlarge

  27. Mouse-Dysferlin-Transcript-Variant2

    Plasmid
    #60215
    Purpose
    Mouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (Dysf Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60215-plasmid-map-sequence-id-96322

    Enlarge

  28. p663-UBC-V5-BioID-STOP_IDG-K

    Plasmid
    #158163
    Purpose
    Gateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-BioID
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    158163-plasmid-map-sequence-id-328021

    Enlarge

  29. R777-E086 Hs.FGFR2-nostop

    Plasmid
    #70370
    Purpose
    Gateway ORF clone of human FGFR2 [NM_000141.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR2 (FGFR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70370-plasmid-map-sequence-id-125819

    Enlarge

  30. pDONR221-CTSS

    Plasmid
    #158445
    Purpose
    Gateway Cloning compatible entry vector for the human CTSS gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSS (CTSS Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158445-plasmid-map-sequence-id-308884

    Enlarge

  35. p667-UBC-hcRed-V5-TurboID_IDG-K

    Plasmid
    #135238
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135238-plasmid-map-sequence-id-268556

    Enlarge

  37. R777-E080 Hs.EXOC7-nostop

    Plasmid
    #70364
    Purpose
    Gateway ORF clone of human EXOC7 [NM_001013839.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC7 (EXOC7 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70364-plasmid-map-sequence-id-125813

    Enlarge

  40. 8061-E08

    Plasmid
    #234245
    Purpose
    Gateway ORF Entry clone of human SMAD2 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SMAD2 (SMAD2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    234245-plasmid-map-sequence-id-469764

    Enlarge

  41. 8061-E23

    Plasmid
    #234260
    Purpose
    Gateway ORF Entry clone of human BMPR2 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    BMPR2 (BMPR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234260-plasmid-map-sequence-id-468859

    Enlarge

  42. 8061-E41

    Plasmid
    #234278
    Purpose
    Gateway ORF Entry clone of mouse Bmpr1a with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Bmpr1a (Bmpr1a Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234278-plasmid-map-sequence-id-469725

    Enlarge

  43. 8061-E47

    Plasmid
    #234284
    Purpose
    Gateway ORF Entry clone of mouse Bmpr2 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Bmpr2 (Bmpr2 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234284-plasmid-map-sequence-id-469719

    Enlarge

  44. 8522-E11

    Plasmid
    #234296
    Purpose
    Gateway ORF Entry clone of human SMAD3 with stop codon (for native or N-terminal fusions); S422A/S423A/S425A mutations (C-terminal phosphorylation sites dead)
    Depositor
    Dominic Esposito
    Insert
    SMAD3 S422A/S423A/S425A (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S422A/S423A/S425A; C-terminal phosphorylation sit…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234296-plasmid-map-sequence-id-468723

    Enlarge

  45. 8522-E19

    Plasmid
    #234299
    Purpose
    Gateway ORF Entry clone of human TGFB3 with stop codon (for native or N-terminal fusions); C227S/C229S mutations
    Depositor
    Dominic Esposito
    Insert
    TGFB3 C227S/C229S (TGFB3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    C227S/C229S
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234299-plasmid-map-sequence-id-468726

    Enlarge

  46. 9336-E24

    Plasmid
    #234307
    Purpose
    Gateway ORF Entry clone of human SMAD3 truncated with stop codon (for native or N-terminal fusions); C-term truncation to delete phosphorylation sites
    Depositor
    Dominic Esposito
    Insert
    SMAD3 truncated (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    del(422-425); C-term truncation to delete phospho…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234307-plasmid-map-sequence-id-469727

    Enlarge

  47. 9336-E25

    Plasmid
    #234308
    Purpose
    Gateway ORF Entry clone of human SMAD3 LSD with stop codon (for native or N-terminal fusions); All four linker sites constitutively active
    Depositor
    Dominic Esposito
    Insert
    SMAD3 LSD (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T179D/S204D/S208D/S213D; All four linker sites co…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234308-plasmid-map-sequence-id-469768

    Enlarge

  48. 10279-E06

    Plasmid
    #234311
    Purpose
    Gateway ORF Entry clone of human TGFBR2 iso1 with stop codon (for native or N-terminal fusions); N-terminal tev cleavage site and E526Q mutation
    Depositor
    Dominic Esposito
    Insert
    tev-Hs TGFBR2 iso1 E526Q (TGFBR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    E526Q
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234311-plasmid-map-sequence-id-468861

    Enlarge

  49. 10279-E07

    Plasmid
    #234312
    Purpose
    Gateway ORF Entry clone of human TGFBR2 iso1 with stop codon (for native or N-terminal fusions); N-terminal tev cleavage site and R537P mutation
    Depositor
    Dominic Esposito
    Insert
    tev-Hs TGFBR2 iso1 R537P (TGFBR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    R537P
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234312-plasmid-map-sequence-id-469767

    Enlarge

  50. pDest_U6_A2_CG2_T1snp_gRNA

    Plasmid
    #213789
    Purpose
    pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA
    Depositor
    William Talbot
    Insert
    nrg1 type I SNP sgRNA (nrg1 Zebrafish)
    Use
    Tol2 destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    213789-plasmid-map-sequence-id-421038

    Enlarge

Showing: 1151 - 1200 of 17982 results