We narrowed to 31,528 results for: Eng;

1. pCI-T7Max-UTR1-T500

   Plasmid

   #178418

   Purpose

   Empty backbone. T7Max promoter

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-T7Max-UTR1-NTerminus8xHis-T500

   Plasmid

   #178421

   Purpose

   Empty backbone. T7Max promoter with N-His Tag

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCTCON2-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158143

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   FAPB2.3.6L1TAG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2-GXB

   Plasmid

   #160926

   Purpose

   Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Inserts

   BFP
   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PHBA03

   Plasmid

   #83537

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas73

   Plasmid

   #82398

   Purpose

   Δccm:Km, used to delete ccm operon

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   ccm deletion
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDS3-aurJ

   Plasmid

   #70152

   Purpose

   Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   aurJ
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD1

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDS2

   Plasmid

   #70135

   Purpose

   CEN/ARS yeast shuttle vector with DS2 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   DS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   AgTEF1

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbB0a-RFP

   Plasmid

   #35371

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBbB0k-RFP

    Plasmid

    #35370

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBbS3c-RFP

    Plasmid

    #35311

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProS

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBbE3c-RFP

    Plasmid

    #35305

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProS

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHB-4

    Plasmid

    #140957

    Purpose

    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105 and tet

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Use

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM6-T7-VHH-GFPminimizer

    Plasmid

    #202488

    Purpose

    Expresses the nanobody GFP minimizer with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP minimizer
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Use

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHB-3

    Plasmid

    #140956

    Purpose

    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LYS-Leu2

    Plasmid

    #218114

    Purpose

    Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH41-Tier1-PhCMV-GLuc

    Plasmid

    #169544

    Purpose

    Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Gaussia Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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