We narrowed to 59,251 results for: cloning

1. p5E-TetO8x-CMVmin (JDW 1087)

   Plasmid

   #224475

   Purpose

   Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TetO(8x)-CMV promoter
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-V5-mScarlet-I_SV40pA (JDW 968)

   Plasmid

   #224529

   Purpose

   A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-DRE-nls (JDW 31)

   Plasmid

   #242561

   Purpose

   Gateway middle entry clone containing Dre recombinase

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   codon optimized DRE-nls
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-Lifeact-mRuby2-3xMyc (JDW 1246)

   Plasmid

   #242554

   Purpose

   Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mRuby2
   

   Use

   Gateway subcloning

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-zFUCCI (JDW 1464)

   Plasmid

   #242558

   Purpose

   Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-H2B-mStayGold-WPRE (JDW 1384)

   Plasmid

   #242550

   Purpose

   Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mStayGold (monomeric StayGold)
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-H2B-mBaoJin-WPRE (JDW 1383)

   Plasmid

   #242549

   Purpose

   Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mBaoJin
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-H2B-mScarlet-3H (JDW 1513)

   Plasmid

   #242551

   Purpose

   Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet3-H
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-mCerulean3-TUBB1A (JDW 1508)

   Plasmid

   #242555

   Purpose

   Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean3-TUBB1A
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-StayGoldc4-Giantin (JDW 1355)

    Plasmid

    #229844

    Purpose

    A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    n2StayGoldc4
    

    Use

    Gateway entry clone

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

    Plasmid

    #224491

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-tdiRFP
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

    Plasmid

    #224524

    Purpose

    A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    rox-3xNLS-mCherry-V5-2xSTOP-rox
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E lexOP-c-fos min pro (JDW 1254)

    Plasmid

    #224477

    Purpose

    Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexOp
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-LexA-mODC (no LexOp) (JDW 937)

    Plasmid

    #224504

    Purpose

    Gateway compatible middle entry clone containing a destabilized LexA transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME nls EGFP-Cre (JDW 1329)

    Plasmid

    #224515

    Purpose

    A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-Cre
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME iCre-I (JDW 1203)

    Plasmid

    #224520

    Purpose

    A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    iCre-I
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-H2A_mCherry_SV40pA (JDW 967)

    Plasmid

    #224528

    Purpose

    A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    H2A-mCherry-SV40pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-V5-mTagBFP2 (JDW 871)

    Plasmid

    #224531

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME H2B mCerulean (JDW 1150)

    Plasmid

    #224543

    Purpose

    A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    H2B-mCerulean
    

    Use

    Gateway cloning

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid

    #214453

    Purpose

    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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