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Showing: 461 - 480 of 1006 results

  1. pcDNA3-RAB-AKARev(T/A)

    Plasmid
    #118479
    Purpose
    Negative-control mutant for RAB-AKARev biosensor.
    Depositor
    Jin Zhang
    Article
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Insert
    RAB-AKARev(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118479-plasmid-map-sequence-id-230370

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  4. pcDNA3-blueCKAR(T/A)

    Plasmid
    #118473
    Purpose
    Negative-control mutant for blueCKAR biosensor.
    Depositor
    Jin Zhang
    Article
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Insert
    blueCKAR(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118473-plasmid-map-sequence-id-230294

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  10. pTL-Bag3-gRNA3

    Plasmid
    #196254
    Purpose
    Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196254-plasmid-map-sequence-id-385944

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  11. pTL-Bag2-gRNA2

    Plasmid
    #196252
    Purpose
    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196252-plasmid-map-sequence-id-385943

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  12. pTL-Bag1-gRNA1

    Plasmid
    #196251
    Purpose
    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196251-plasmid-map-sequence-id-385941

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  13. pTL-Bag4-gRNA4

    Plasmid
    #196255
    Purpose
    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196255-plasmid-map-sequence-id-385931

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  15. MC177VSG221RNAi

    Plasmid
    #59730
    Purpose
    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
    Depositor
    Gloria Rudenko
    Article
    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10
    Insert
    VSG221 RNAi fragment
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    opposing T7 promoters
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59730-plasmid-map-sequence-id-93746

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  17. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70659-plasmid-map-sequence-id-162503

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Showing: 461 - 480 of 1006 results