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pCDNA4/TO-SPOP-myc (W22R)

Plasmid

#197004

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

W22R

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (E47K)

Plasmid

#197003

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

E47K

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W131G SPOP 1-374

Plasmid

#197019

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W131G

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E78K SPOP 1-374

Plasmid

#197018

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E78K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E47K SPOP 1-374

Plasmid

#197017

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E47K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W22R SPOP 1-374

Plasmid

#197016

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W22R

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS09

Plasmid

#52649

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS09

Expression

Mammalian

Mutation

L414T mutated from GCaMP3

Promoter

T7

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only
pBacPak-HA-CG9866-1-200

Plasmid

#52291

Purpose

Expresses CG9866 residues 1-200

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS05

Plasmid

#52646

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS05

Expression

Mammalian

Mutation

M373Q mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS08

Plasmid

#52648

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS08

Expression

Mammalian

Mutation

T412Q/L414T mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-OTUB1 C91S-pcDNA3.1

Plasmid

#118210

Purpose

Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS9

Plasmid

#111765

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47-Mango II

Plasmid

#127586

Purpose

Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA with Mango II tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Cytosolic FAK biosensor

Plasmid

#78300

Purpose

FRET biosensor. Visualization of cytosolic FAK activity in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Cytosolic FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Control

Plasmid

#127582

Purpose

Expresses 5S-F30-Control rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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