We narrowed to 8,767 results for: PAN

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pJT139_GalL_nCDA1-VRERBE3

Plasmid

#145102

Purpose

Expressing base editor nCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-VRERBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMtb MCM

Plasmid

#138296

Purpose

Expression of Mtb MCM small and large subunit

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Inserts

mutA
mutB

Tags

His tag and TEV cleavage site

Expression

Bacterial

Mutation

inserted TEV cleavage site between His-tag and m…

Promoter

T7

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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A3H HapII R175/6E-Cas9n-UGI

Plasmid

#119142

Purpose

Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI

Use

CRISPR

Mutation

A3H Hap II RR175/6EE

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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SP65U1-135a-1

Plasmid

#105819

Purpose

plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid

Depositor

Cecilia Mannironi

Article

Mannironi et al PLoS One. 2013 Sep 4;8(9):e73385. doi: 1

Insert

MIRNA135A1 (Mir135a-1 Mouse)

Expression

Mammalian

Promoter

U1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PsuACR_433-EYFP

Plasmid

#103529

Purpose

Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin PsuACR_433

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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p-miR-SC

Plasmid

#65055

Purpose

Expression of miR148a with scrambled seed in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (mutated) (MIR148A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-WW-EF1+EF2

Plasmid

#18080

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
HsCLYBL(aa23-340)-His

Plasmid

#111288

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)

Tags

His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2GFP

Plasmid

#82416

Purpose

3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

GFP

Use

CRISPR and Lentiviral

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

Plasmid

#74309

Purpose

Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-neo:β2-365AAQA368 L349M

Plasmid

#35610

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 L349M 365AAQA368 (Chrnb2 Mouse, mouse strain 129)

Expression

Mammalian

Mutation

L349M and 365AAQA368

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi G418R mbIL21

Plasmid

#194329

Purpose

Consitiutive mammalian expression of membrane bound human IL-21

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

mbIL-21 (IL21 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Article

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p221- purVSG117UTR

Plasmid

#59732

Purpose

Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

Depositor

Gloria Rudenko

Article

Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

Insert

VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site

Use

For homologous recombination into vsg221 expressi…

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-bHs

Plasmid

#134259

Purpose

For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

Depositor

Edwin Manuel

Article

Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

Insert

Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium

Tags

6x His

Expression

Bacterial

Mutation

codon optimized for Salmonella typhimurium

Promoter

AraC

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRM27

Plasmid

#163695

Purpose

ubb promoter driven expression of lck:mNeonGreen

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

lck:mNeonGreen (lck Zebrafish)

Use

Zebrafish expression

Tags

mNeonGreen

Promoter

ubb

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/empty

Plasmid

#105505

Purpose

Empty backbone of MSCV-driven retroviral cDNA expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Type

Empty backbone

Use

Retroviral

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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