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pcDNA3 HRPT2 136X

Plasmid

#11049

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 136X (CDC73 Human)

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-FH-Nsp15

Plasmid

#157721

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp15 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX p85b(C)-SH2

Plasmid

#46466

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(C) (PIK3R2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 610-728

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Socs4-SH2

Plasmid

#46507

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs4 (SOCS6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 376-498

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp7

Plasmid

#157689

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp7 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX Zap70(N)-SH2

Plasmid

#46534

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(N) (ZAP70 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-116

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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CRY-tTA (B702)

Plasmid

#92032

Purpose

Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

tTA2 (TetR fused to VP64)

Expression

Mammalian

Mutation

NLS on CRY2 is mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp15-HF

Plasmid

#157722

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX Jak2-SH2

Plasmid

#46448

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Jak2 (JAK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 394-497

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grb14-SH2

Plasmid

#46439

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb14 (GRB14 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 427-540

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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LK4-Ets2

Plasmid

#44606

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 (Ets2 Mouse)

Expression

Mammalian

Promoter

human Beta-Actin

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
FSW-HRP-V5-MDGA1

Plasmid

#82541

Purpose

Lentiviral vector for expressing HRP-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 227X

Plasmid

#11050

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 227X (CDC73 Human)

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-TPD53

Plasmid

#172457

Purpose

Expression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD53 (TPD52L1 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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