We narrowed to 22,280 results for: fus

1. pAs-Cpf1-pGL3-U6-sgRNA

   Plasmid

   #107683

   Purpose

   Expresses AsCpf1 crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK390(Stag-3FLAG-BSD)

   Plasmid

   #121191

   Purpose

   C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Stag-3FLAG-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-Flag-TZAP

   Plasmid

   #87176

   Purpose

   retroviral stable expression of Flag-TZAP

   Depositor

   Eros Lazzerini Denchi

   Article

   Li et al Science. 2017 Jan 12. pii: aah6752. doi:

   Insert

   TZAP (ZBTB48 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti X2 Puro Empty (w609-1)

   Plasmid

   #20957

   Purpose

   3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Puro

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR/pTER shLUC (w177-1)

   Plasmid

   #17472

   Purpose

   Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase shRNA
   

   Use

   RNAi; Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRESneo-mTOR-RR

   Plasmid

   #31075

   Depositor

   Jie Chen

   Article

   Park et al J Biol Chem. 2005 Sep 9. 280(36):32009-1

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S2035T

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pGBW-m4134904

   Plasmid

   #151930

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d1-15 d1209-1273 R682A R685G K986P V987P

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pENTR1A-NTAP-C (w324-1)

   Plasmid

   #17404

   Purpose

   Gateway entry vector for NTAP (N-terminal affinity purification), frame C

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   CBP and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PABE-1

   Plasmid

   #115622

   Purpose

   Targeted A to G in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   NLS-wtTadA-TadA7.10-nCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMK389 (mCherry-BSD)

    Plasmid

    #121190

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR1A-NTAP-B (w323-1)

    Plasmid

    #17403

    Purpose

    Gateway entry vector for NTAP (N-terminal affinity purification), frame B

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    CBP and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF:iCas9

    Plasmid

    #138267

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in human.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cas9-D10A, H840A

    Promoter

    Ef1a

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK1036

    Plasmid

    #17728

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    NusG (nusG Aquifex aeolicus)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-PH-GAP1IP4BP

    Plasmid

    #20200

    Depositor

    Robin Irvine

    Article

    Hammond et al J Cell Biol. 2009 Jan 19. ():.

    Insert

    PH-GAP1IP4BP (RASA3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Isolated PH/Btk domain

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19/N-eCFP

    Plasmid

    #183155

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR4-FKBP12DD N-term B (w385-4)

    Plasmid

    #17415

    Purpose

    Gateway entry vector for Degron, N-term, frame B

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    FKBP12DD (L106P)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sv272-HisMBP-TZAPZNF9-11

    Plasmid

    #87185

    Purpose

    bacterial expression of HisMBP-TZAPZNF9-11

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    Tags

    HisMBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK388 (mClover-BSD)

    Plasmid

    #121189

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mClover-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST1374-Lb-Cpf1-NLS

    Plasmid

    #107680

    Purpose

    Expresses LbCpf1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    LbCpf1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR/pTER shEGFP #1 (628-2)

    Plasmid

    #17470

    Purpose

    Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Enhanced green fluorescent protein shRNA #1
    

    Use

    RNAi; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYC44

    Plasmid

    #63903

    Purpose

    Initial Integrative Vector

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    FRT::3'UTRcta::NAT::FRT

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC19/mChe-C

    Plasmid

    #183160

    Purpose

    Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ECYFP

    Plasmid

    #111204

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2001 Feb 9;276(6):3805-10.

    Insert

    ECFP-Xpress tag-GFP10C fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJEC578

    Plasmid

    #174366

    Purpose

    dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    dCas9 fused to SYNZIP18
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23150

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

    Plasmid

    #49313

    Purpose

    Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Insert

    nls-mCherry-Flag
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQCXP CMV/TO empty (w368-1)

    Plasmid

    #17393

    Purpose

    Retroviral Empty control vector, CMV/TO promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYC12

    Plasmid

    #63911

    Purpose

    Initial Overexpressing Vector

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHu6-gRNA-NT1

    Plasmid

    #81203

    Purpose

    hU6 expression of gRNA NT1

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA NT1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Venus-7aa-Cb5-pEGFP-C1

    Plasmid

    #166764

    Purpose

    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5a Rat)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EZ-L498

    Plasmid

    #131771

    Purpose

    PPGK1_FUSN_Citrine _PixE_TCYC1

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PPGK1_FUSN_Citrine _PixE_TCYC1
    

    Use

    Integration into his3 locus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge