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pMGIB-HA-hTCAB1

Plasmid

#167460

Purpose

Retroviral expression of HA-TCAB1 (WDR79) in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

TCAB1 (WRAP53 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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LXSN-Cx43

Plasmid

#60692

Purpose

retroviral expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_ACR_Trypsin

Plasmid

#110042

Purpose

Protein expression and purification of ACR_Trypsin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ACR_Trypsin (ACR Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2P1104A-VSV

Plasmid

#139350

Purpose

Expression of the TYK2-P1104A variant, rs34536443

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-P1104A

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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ADGRL3-CTF-Flag

Plasmid

#201219

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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MO70-hCRACR2A-isoform-c

Plasmid

#79591

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

Insert

CRACR2A-isoform-c (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pSMP-Ehmt1_4

Plasmid

#36338

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

Plasmid

#83461

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, R4113A

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pX459_HR_Prnp_3

Plasmid

#78623

Purpose

pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pETM33_CEP55_EABR

Plasmid

#178469

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CEP55_EABR (CEP55 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSMP-Ehmt1_1

Plasmid

#36335

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe JunD-Venus puro

Plasmid

#58496

Purpose

mammalian expression of Venus-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
TIA1 RRM-CFP-FRBx5

Plasmid

#103780

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pETM33_EPS15_EH

Plasmid

#178475

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EPS15_EH (EPS15 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSMP-MeCP2_1

Plasmid

#36377

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MeCP2 (MECP2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
His-ZZ-TEV-BICDR1

Plasmid

#111859

Purpose

Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Tags

His-tag, TEV site, and ZZ

Expression

Insect

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp

Plasmid

#108481

Purpose

inducible expression of vsv-CenpB-1-166-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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