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pMST665_BB3_L 23_gRNAempty_cas9_BbsI

Plasmid

#90278

Purpose

BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

Cas9

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pZS2katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78213

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBN100

Plasmid

#35106

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

caURA3PR

Use

Yeast replicating

Available Since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAL10

Plasmid

#236006

Purpose

Backbone for assembling gRNA arrays with LacZ gene for blue white screening

Depositor

Carl de Boer

Article

Salaudeen et al Synth Biol (Oxf). 2025 Dec 18;11(1):ysaf

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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YIplac211-Sys1-HaloTagx2

Plasmid

#247751

Purpose

Insert a C-terminal HaloTagx2 at SYS1 by popin-popout

Depositor

Benjamin Glick

Insert

Sys1-HaloTagx2

Expression

Yeast

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-BCAT1

Plasmid

#240021

Purpose

Expresses human BCAT1 fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

BCAT1

Use

Lentiviral

Tags

Flag

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pRsetB-RoTq-Off

Plasmid

#234466

Purpose

Bacterial expression vector for RoTq-Off

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

RoTq-Off

Tags

7xHis

Expression

Bacterial

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pR6K-Donor(PmcCAST)_KanR

Plasmid

#224928

Purpose

Transposition assay

Depositor

Leifu Chang

Article

Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

Insert

LE_cargo_RE

Expression

Bacterial

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK191

Plasmid

#226658

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAC12

Plasmid

#197406

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pBBR1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pBBR1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC09

Plasmid

#197403

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and RSF1010 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RESF1010 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Diffusive 54kDa

Plasmid

#201337

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-4PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Diffusive 47kDa

Plasmid

#201336

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-3PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMCL9

Plasmid

#176186

Purpose

2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI1

Plasmid

#176155

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI5

Plasmid

#176158

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII2

Plasmid

#176160

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAGT572_Nemo

Plasmid

#153487

Purpose

Level 1 episomal expression plasmid for S. cerevisiae

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DUET-Prp18

Plasmid

#111354

Purpose

protein purification

Depositor

Christine Guthrie

Insert

PRP18

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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