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pdelta105-ABCb10-GFP

Plasmid

#19034

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

ABCb10 lacking the mitochondrial targetting sequence

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pTHSSd_40

Plasmid

#59963

Purpose

High core fragment expression without SynZIP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment lacking SynZIP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

Plasmid

#209090

Purpose

mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔEC1ΔICD-EB

Plasmid

#208522

Purpose

EBFP2-fused PcdhγB2 lacking an EC1 domain and an ICD domain

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

EBFP2

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔEC1ΔICD-mCh

Plasmid

#208521

Purpose

mCherry-fused PcdhγB2 lacking an EC1 domain and an ICD domain

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHACK-QF2-AgU6-mCherry

Plasmid

#184530

Purpose

HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

Depositor

Christopher Potter

Article

Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

Insert

QF2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Anopheles U6 promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-Ugi1-11

Plasmid

#197690

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ugi1 enhancer-blocking insulator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-Ugi3-11

Plasmid

#197691

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ugi3 enhancer-blocking insulator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-Ugi4-11

Plasmid

#197692

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ugi4 enhancer-blocking insulator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc15

Plasmid

#198284

Purpose

Produces PVCs lacking payloads by elimination of the payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1(1k + 50 bp)::Venus

Plasmid

#117421

Purpose

For analysis of AIB cell differentiation by tracking Venus fluorescence under the expression of Pinx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

Venus

Use

Tags

Expression

Worm

Mutation

Promoter

inx-1

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pKB236.1

Plasmid

#170028

Purpose

Encodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
TCP Δ C-terminal alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Availability

Academic Institutions and Nonprofits only

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pAXEF-Rap1-WT

Plasmid

#32697

Depositor

Vicki Boussiotis

Article

Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

Insert

RAP1 (RAP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF promoter

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAW62.YY1.FKBP.knock-in.mCherry

Plasmid

#104370

Purpose

For knocking in FKBP F36V P2A mCherry onto YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAW63.YY1.FKBP.knock-in.BFP

Plasmid

#104371

Purpose

For knocking in FKBP F36V P2A BFP

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-BFP

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-EGFP-GPI

Plasmid

#65294

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge