Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,724 results for: Pol

Clear keywords
Showing: 4841 - 4860 of 7724 results

  1. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162670-plasmid-map-sequence-id-321452

    Enlarge

  4. pJin-239

    Plasmid
    #125716
    Purpose
    ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1
    Depositor
    Bryan Dickinson
    Publication
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
    Expression
    Mammalian
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125716-plasmid-map-sequence-id-244343

    Enlarge

  6. pOCC194

    Plasmid
    #118882
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Tags
    SNAP tag, cleavable with TEV protease, HIS6, clea…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118882-plasmid-map-sequence-id-232359

    Enlarge

  7. pOCC231

    Plasmid
    #118885
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and mCitrine…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118885-plasmid-map-sequence-id-232309

    Enlarge

  8. pOCC254

    Plasmid
    #118889
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; mCherry, cl…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118889-plasmid-map-sequence-id-232286

    Enlarge

  10. MTHFR (MTHFRA-c042)

    Plasmid
    #110271
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MTHFR (MTHFR Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110271-plasmid-map-sequence-id-212270

    Enlarge

  11. GST-PC-2-CTS

    Plasmid
    #86068
    Purpose
    A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Polycystin-2 (Pkd2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains the region from 1-15
    Promoter
    tac
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86068-plasmid-map-sequence-id-177978

    Enlarge

  12. pHT-PRD

    Plasmid
    #87755
    Purpose
    Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)
    Depositor
    Yu-Wen Hwang
    Insert
    Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
    Tags
    6X histidine tag
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87755-plasmid-map-sequence-id-173677

    Enlarge

  14. pMpGWB425

    Plasmid
    #68690
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Publication
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Triple repeats of Citrine (3xCitrine)
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68690-plasmid-map-sequence-id-122052

    Enlarge

Showing: 4841 - 4860 of 7724 results