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Addgene

We narrowed to 10,650 results for: cat.1

Showing: 4841 - 4860 of 10650 results
  1. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid
    #105448
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector
    Depositor
    Has Service
    AAV9
    Insert
    ChrimsonR-mRuby2-ST
    Use
    AAV
    Tags
    mRuby2 and soma targeting motif
    Expression
    Mutation
    K176R in Chrimson
    Promoter
    hSyn
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 4841 - 4860 of 10650 results