We narrowed to 10,699 results for: cat.1

1. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

   Plasmid

   #71213

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
   

   Use

   Tags

   dSpCas9-BFP-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-HA-RAD52-∆C

   Plasmid

   #235677

   Purpose

   Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 302-410

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRIPZ-CTID195-GSQ-UBnc-PURO

   Plasmid

   #208037

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   Ubnc (UBC Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Expression

   Mutation

   L73P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-ARAP3(R307,8A)

   Plasmid

   #39486

   Depositor

   Sonja Vermeren

   Article

   Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

   Insert

   ARAP3(R307,8A) (ARAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R307,8A = PH domain mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-ARAP3(C504A)

   Plasmid

   #39485

   Depositor

   Sonja Vermeren

   Article

   Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

   Insert

   ARAP3(C504A) (ARAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C504A = Arf GAP domain mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCMVtrunc LifeactΔC4-msfGFPΔN7

   Plasmid

   #231555

   Purpose

   Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMVtrunc msfGFPΔC9-LifeactΔN2

   Plasmid

   #231556

   Purpose

   Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Δ1-60MEK1C222

   Plasmid

   #164641

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S222C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Δ1-60MEK1C218

   Plasmid

   #164643

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Δ1-60MEK1C218C222

    Plasmid

    #164645

    Purpose

    Bacterial expression plasmid for His6-ΔMEK1C218C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    S218C/S222C/C277S/C376S; residues 1-60 at N-termi…

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Fuw-dCas9-Tet1CD_IM

    Plasmid

    #84479

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

14. Fuw-dCas9-Dnmt3a_IM

    Plasmid

    #84478

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DCIP

    Plasmid

    #193860

    Purpose

    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    NLS-mCherry-GFP1-10
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOZITX-S1

    Plasmid

    #184925

    Purpose

    Expresses the catalytic S1 subunit of OZITX (EcPltAB)

    Depositor

    Jonathan Javitch

    Article

    Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

    Insert

    codon optimized S1 subunit of OZITX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPU-huTRIMmiR30-TRIM5

    Plasmid

    #132938

    Purpose

    All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    human TRIM5
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid

    #171660

    Purpose

    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBintT

    Plasmid

    #48134

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge