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pFUW mTagBFP2-P2A-NeoR

Plasmid

#239568

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mito-GRX1-roGFP2

Plasmid

#239561

Purpose

Expression and mitochondrial localization of Grx1-roGFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

roGFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mTagBFP2-P2A-PuroR

Plasmid

#239565

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW H2B-mCherry-P2A-PuroR

Plasmid

#239554

Purpose

Expression and nuclear localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-Floxed-FlpO-2A-H2BV5c.o.,rev.-WPRE

Plasmid

#234894

Purpose

CAG-driven, Cre-dependent FlpO recombinase for H2B-V5

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

Floxed-FlpO-2A-H2BV5

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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KL060 - pBlueScript II SK-mAID-EGFP CTCF donor

Plasmid

#232926

Purpose

Donor plasmid for tagging mouse CTCF with mAID-eGFP at the N-terminus. Used with KL061 - PX330-sgRNA006_CTCF sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

mAID-eGFP with mCTCF homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::QF+hGR

Plasmid

#232611

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::CHRIMSON_CE

Plasmid

#232603

Purpose

Pan-neuronal expression of opsin 'Chrimson', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Chrimson

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS+Δpes-10P::AI::eTsChR

Plasmid

#232612

Purpose

Expression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

eTsChR

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::Voltron

Plasmid

#232602

Purpose

Pan-neuronal expression of voltage sensor 'Voltron', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Voltron

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::TsChR

Plasmid

#232604

Purpose

Pan-neuronal expression of opsin 'TsChR', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

TsChR

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCCG_nit9fl_random_linker

Plasmid

#228418

Purpose

Expression of NIT-9 with moco resulfurase linkage region in N. crassa at the his-3 locus

Depositor

Robert Haensch

Insert

NIT-9 (GPHN Human)

Use

Protein expression in n. crassa

Mutation

removal of the linkage region and insertion of th…

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaE

Plasmid

#228826

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaE

Tags

eGFP

Expression

Mammalian

Mutation

aa 49-494

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC

Plasmid

#228824

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaA

Plasmid

#228823

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaA

Tags

eGFP

Expression

Mammalian

Mutation

aa 25-1216

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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BALF5-Y753F

Plasmid

#192456

Purpose

Expresses BALF5 with Y753F mutation

Depositor

Bill Sugden

Article

Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

Insert

BALF5 (Y753F) (BALF5 Epstein-Barr Virus)

Tags

FLAG

Expression

Mammalian

Mutation

S758T

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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BALF4-S758T

Plasmid

#192457

Purpose

Expresses BALF5 with S758T mutation

Depositor

Bill Sugden

Article

Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

Insert

BALF5 (S758T) (BALF5 Epstein-Barr Virus)

Tags

FLAG

Expression

Mammalian

Mutation

S758T

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD 16S rRNA-2

Plasmid

#210834

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD 16S rRNA-1

Plasmid

#210833

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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