We narrowed to 11,748 results for: pcDNA

1. pcDNA 3.1 SIRT1-deltaExon2

   Plasmid

   #105671

   Purpose

   Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2/V5 hsa-mir-483

   Plasmid

   #26315

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-483 (MIR483 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-FLAG-HA-CAD S1900A

   Plasmid

   #46240

   Purpose

   FLAG-HA-CAD with S1900 mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1900 changed to Alanine (S1900A)

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3-HA-human MYCN-V421D

   Plasmid

   #74166

   Purpose

   Mammalian expression of human MycN-VD mutant

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MycN (MYCN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   V421D

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. Nano-lantern-peroxisome/pcDNA3

   Plasmid

   #51974

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   SKL

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3.1(-) + SLNCR1 _701-902

   Plasmid

   #86821

   Purpose

   mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3-Flag-SPRTN-Y117C

   Plasmid

   #110216

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y117C, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1+_LN-HA-AGO2-5xE

   Plasmid

   #92011

   Purpose

   Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   Lambda-N-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, T830E, S831E, S834E (5xE)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Flag MFF iso4 WT

   Plasmid

   #74390

   Purpose

   Flag tagged human MFF isoform 4

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA GAL4 CREB 1-341 M1

    Plasmid

    #22973

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB (Creb1 Rat, GAL4-fusion)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine of CREB

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human NCKX2-AS-long (pcDNA3.1+)

    Plasmid

    #75201

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

    Plasmid

    #181842

    Purpose

    EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only residues 12-112 of PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-REDD1 P139A (#625)

    Plasmid

    #99945

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P139A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 9E10.ChWT.Gal4.pCDNA1/AMP

    Plasmid

    #21914

    Depositor

    David Ron

    Article

    Wang et al Science. 1996 May 31. 272(5266):1347-9.

    Insert

    CHOP (Ddit3 Mouse)
    

    Use

    Tags

    GAL4-DNA-BD and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA HA HP1 gamma delta C

    Plasmid

    #24081

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Missing C-terminal 25 amino acids

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-HA-REDD1 S137A (#627)

    Plasmid

    #99943

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S137A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3_PKA-CαN(E12,14K)-mPAGFP

    Plasmid

    #168495

    Purpose

    Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5 FRT/TO MycLAP-STIL ∆CC

    Plasmid

    #80268

    Purpose

    mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    missing conserved coiled-coil (amino acids 721-74…

    Promoter

    CMV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/nV5-DEST-Apr1

    Plasmid

    #20135

    Depositor

    Valeri Vasioukhin

    Article

    Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

    Insert

    pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-p160(400-580)

    Plasmid

    #45

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses only aa400-580

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only