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Plasmid#123920PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_3
Plasmid#103949PurposeProtein expression and purification of X104 PDZ3 domainDepositorInsertTJP2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_2
Plasmid#103955PurposeProtein expression and purification of X104 PDZ2 domainDepositorInsertTJP2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK0_046
Plasmid#123976PurposeEncodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemhDepositorInserthThal5.10-2_hg18 CAS9 Destination - HygroR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0419v
Plasmid#124021PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T,LacI-T]
Plasmid#60768PurposeContains PIq driving expression FruR-T, and PI driving expression of LacI-T.DepositorInsertsFruR-T
Dimeric LacI with the TAN DBD
UseSynthetic BiologyTagsExpressionBacterialMutationDimeric LacI with the TAN DBD. and LacI/GalR repr…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T,RbsR-T]
Plasmid#60773PurposeContains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with GalS LB…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEa
Plasmid#121900PurposePlasmid for making zebrafish apoEa in situ probeDepositorInsertApoEa (apoea Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
Plasmid#139482PurposeExpresses PhyB621-mCherry-HRasCT in mammalian cells.DepositorInsertPhyB621 (PHYB Mustard Weed)
UseLentiviralTagsmCherry-HRasCTExpressionMammalianMutationG229L and deletion of aa Y at position 235 in mch…PromoterTet responsible elementAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ROZA-XL YF
Plasmid#64195PurposeFluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequenceDepositorInsertROZA-XL YF
UseTagsExpressionMammalianMutationROZA-XL tyrosine phosphorylation site mutated to …PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Hes3
Plasmid#61535PurposeExpresses Hes3 in mammalian cellsDepositorInsertHes3 (Hes3 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_7
Plasmid#103891PurposeProtein expression and purification of Multiple PDZ protein PDZ7 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_13
Plasmid#103897PurposeProtein expression and purification of Multiple PDZ protein PDZ13 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_3
Plasmid#103887PurposeProtein expression and purification of Multiple PDZ protein PDZ3 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
NC1 pGLUE WTX (1-804)
Plasmid#36956DepositorInsertWTX (AMER1 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-FLAG-Neuroserpin oloop
Plasmid#58262PurposeRetroviral vector for expressing mutated Neuroserpin in mammalian cellsDepositorInsertNeuroserpin (SERPINI1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationMutant of neuroserpin lacking five residues from …PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPKm-231
Plasmid#90500PurposepSIN - EF-1alpha - MTS - tFd - P2A - MTS - tFNR, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd) and Ferredoxin-NADP(+) oxireductase (FNR), under EF-1alpha promoterDepositorInsertmito-tFD and mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-232
Plasmid#90501PurposepSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoterDepositorInsertmito-tHO1 and mito-tPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-234
Plasmid#90507PurposepSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertmito-sHO1, mito-sPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T,TreR-T]
Plasmid#60775PurposeContains PIq driving expression GalS-T, and PI driving expression of TreR-T.DepositorInsertsGalS-T
TreR-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with GalS LB…PromoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T,TreR-T]
Plasmid#60772PurposeContains PIq driving expression FruR-T, and PIq driving expression of TreR-T.DepositorInsertsFruR-T
TreR-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with FruR LB…PromoterAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-T,TreR-T]
Plasmid#60776PurposeContains PIq driving expression RbsR-T, and PI driving expression of TreR-T.DepositorInsertsRbsR-L
TreR-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with RbsR LB…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-T,LacI-T]
Plasmid#60767PurposeContains PIq driving expression RbsR-T, and PI driving expression of TreR-L.DepositorInsertsRbsR-L
Dimeric LacI with the TAN DBD
UseSynthetic BiologyTagsExpressionBacterialMutationDimeric LacI with the TAN DBD. and LacI/GalR repr…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL1
Plasmid#41835DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-CKAR2
Plasmid#236100PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.DepositorInsertLAMP1-ExRai-CKAR2 (LAMP1 Synthetic, Human)
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Par6-ExRai-CKAR2
Plasmid#236101PurposeSubcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.DepositorInsertPar6C-ExRai-CKAR2 (PARD6A Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GPHN-FingR-mGold-CCR5TC
Plasmid#231784PurposeVisualization of inhibitory post-synapse using GPHN-FingR-mGoldDepositorInsertGPHN-FingR-mGold-CCR5TC
UseTagsExpressionMammalianMutationPromoterActin promoter with CMV enhancerAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-YR-ICUE-mVenus
Plasmid#231787PurposeVisualization of cAMP dynamics using YR-ICUE-mVenusDepositorInsertYR-ICUE-mVenus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only