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pTA1cI-T3

Plasmid

#193789

Purpose

Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Truncated version of Lambda CI, named T3, where the first two codons are deleted

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLexA::SRDX (GB0732)

Plasmid

#68184

Purpose

Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LexA Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGal4BD::BDR (GB0734)

Plasmid

#68185

Purpose

Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Gal4 Binding Domain fused to BDR Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Alpha-0 Dronpa

Plasmid

#44193

Depositor

Narasimhan Gautam

Article

Chisari et al J Biol Chem. 2007 Aug 17;282(33):24092-8

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

Dronpa

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y20,27A

Plasmid

#58465

Purpose

Expresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosines 20 and 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y27A

Plasmid

#58464

Purpose

Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF9-2xCSTREP

Plasmid

#136173

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF9

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF9

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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B*58:01:01

Plasmid

#203261

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*58:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*24:02:01

Plasmid

#203295

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:02:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*46:01:01

Plasmid

#203250

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*46:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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A*11:01:01:01

Plasmid

#203276

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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B*40:01:01

Plasmid

#203257

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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A*02:06:01

Plasmid

#203279

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:06:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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A*33:03:01

Plasmid

#203275

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:03:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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A*11:02:01

Plasmid

#203282

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:02:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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B*50:01:01

Plasmid

#203234

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*50:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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B*40:02:01

Plasmid

#203239

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:02:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF57-2xCSTREP

Plasmid

#136217

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF57

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaSpCas9-NLS-6xHis

Plasmid

#207386

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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