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pGBW-m4046795

Plasmid

#145598

Purpose

Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE146

Plasmid

#118498

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLQ-KO-tgt50

Plasmid

#126578

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB131

Plasmid

#68585

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-K55-HilA

Plasmid

#153329

Purpose

Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K55 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB235

Plasmid

#68626

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB228

Plasmid

#68619

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pPMW-attB

Plasmid

#61814

Purpose

pUASp plasmid with attB for Drosophila transgenesis

Depositor

Frederique Peronnet

Article

Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

Type

Empty backbone

Use

Tags

6xMyc

Expression

Insect

Mutation

Promoter

UASp

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB323

Plasmid

#68651

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB407

Plasmid

#68672

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE163

Plasmid

#118511

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB331

Plasmid

#68659

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G04

Plasmid

#67138

Purpose

Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and HIS

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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IQ013: pMVP/Ad-DEST

Plasmid

#121843

Purpose

pMVP destination vector, empty adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Article

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Use

Tags

12X MS2 stem loops

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB106

Plasmid

#68560

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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