We narrowed to 51,827 results for: ANG

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pTT5-hSDC1-2

Plasmid

#52378

Purpose

expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

GAGAL of syndecan-1 replaced with of GADED of SDC…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pLY164

Plasmid

#184140

Purpose

Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-R3, PrhaB, and Ptet

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLY305

Plasmid

#184159

Purpose

Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
mutsfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

K140N, Mutations D10A and H840A on WT cas9. Synon…

Promoter

Pcon, PpspA-zraP-22, PrhaB, and Ptet

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1-4

Plasmid

#52375

Purpose

expresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

GAGAL of syndecan-1 replaced with GDLDD of SDC4; …

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pTW334e2b

Plasmid

#246587

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Mutation

AtBSD2W108A/L109E

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e4

Plasmid

#246589

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, RbcX2, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e2

Plasmid

#246586

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-YFP-Rb-IRES-blasticidin

Plasmid

#223964

Purpose

Fluorescent reporter with exogenous Rb

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-YFP-Rb (RB1, H2B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPTapp

Plasmid

#236000

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPTapp (PLEKHA1, LAMP1 Synthetic, Human)

Tags

Citrine, Full-length LAMP1, and truncECFP

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-INPP5E

Plasmid

#236004

Purpose

Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-INPP5E (LAMP1, INPP5E Synthetic, Human)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#1-puro

Plasmid

#231984

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

Plasmid

#213522

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed V290 to A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

Plasmid

#213523

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed N306 to K

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

Plasmid

#221896

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH0994-pOCC177-Fus-wt_(1-211)

Plasmid

#221886

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

PLD domain (aa 1-211)

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt

Plasmid

#221890

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

HNRNPA3 (HNRNPA3 Human)

Tags

6xHis-MBP-PreScission-mGFP-TEV

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2 (1-416) (K57A)

Plasmid

#197124

Purpose

Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

Insert

TAOK1 (aa1-416, K57A) (TAOK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A and deleted C-terminus

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-UbC-3xNLS-mScarlet-I

Plasmid

#191099

Purpose

To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-mScarlet-I

Use

AAV and Synthetic Biology

Tags

Three repeats of the nuclear localizzation seque…

Expression

Mammalian

Mutation

optimized to human codon usage

Promoter

UbC

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLY315

Plasmid

#184160

Purpose

Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-zntA-1034, PrhaB, and Ptet

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY162

Plasmid

#184138

Purpose

Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-R1, PrhaB, and Ptet

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY163

Plasmid

#184141

Purpose

Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-R2, PrhaB, and Ptet

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY168

Plasmid

#184142

Purpose

Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-Ar2, PrhaB, and Ptet

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY300

Plasmid

#184158

Purpose

Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-oxyS-86, PrhaB, and Ptet

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY320

Plasmid

#184161

Purpose

Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Inserts

dCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS

Use

Synthetic Biology

Tags

ASV tag and λN22plus

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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px458-AMBRA1 (optimized for N-terminal knock-in)

Plasmid

#172608

Purpose

Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

Plasmid

#128666

Purpose

mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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