We narrowed to 5,822 results for: pCas

1. pZeo-Cas9-gate

   Plasmid

   #113744

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRvCAHS3-mEGFP

   Plasmid

   #205008

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRvACT-mCherry

   Plasmid

   #205002

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330-UFL1 sgRNA1

   Plasmid

   #134635

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA1 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-ATG13

   Plasmid

   #171413

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG13 (ATG13 Synthetic)
   

   Expression

   Mammalian

   Available Since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP43 NOTAG3

   Plasmid

   #28208

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   aa170-414 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pJK01_Rho_minprox_DsREd

   Plasmid

   #173489

   Purpose

   PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

   Depositor

   Barak Cohen

   Article

   White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Insert

   Mouse Rhodopsin promoter driving DsRed reporter gene
   

   Expression

   Mammalian

   Promoter

   Mouse Rhodopsin

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-TUBB4B

   Plasmid

   #207785

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Double UP mNeon to mScarlet Rac1-N17

   Plasmid

   #125137

   Purpose

   Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).

   Depositor

   Erik Dent

   Article

   Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

   Insert

   mNeon/mScarletP2A Rac1-N17
   

   Expression

   Mammalian

   Mutation

   Removed Not1 site in mScarlet. 17th AA in Rac1 ch…

   Promoter

   CAG

   Available Since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CP1028-ABE8e-dimer

    Plasmid

    #138498

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE80L TriCatcher-RGDSP-MMP

    Plasmid

    #112633

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP-MMP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. px459-RhebL1 sgRNA

    Plasmid

    #133769

    Purpose

    Expresses Cas9 and human RhebL1 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    RhebL1 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAtCas9-D10A_2

    Plasmid

    #91160

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-gp78

    Plasmid

    #61833

    Purpose

    to make gp78 Knockout cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. L40C-CRISPR.EFS.PAC

    Plasmid

    #89393

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFL-mPML-3KRmut-iB

    Plasmid

    #140287

    Purpose

    Expression of mouse PML KR mutant

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    PML (Pml Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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