We narrowed to 7,294 results for: 11

1. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

   Plasmid

   #114087

   Purpose

   U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for FnCas12a crRNAs
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

   Plasmid

   #162098

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to HA-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   HA-FLAG-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

   Plasmid

   #162099

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-ProtC

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FCK-Chronos-GFP

   Plasmid

   #80902

   Purpose

   Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEN25-Ub Hs

   Plasmid

   #83232

   Purpose

   pHis-par2-Ub Hs

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Ubiqutin (UBC Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuro-Gcn5(D608A)

   Plasmid

   #63707

   Purpose

   retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   D608A mutation

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

   Plasmid

   #114089

   Purpose

   CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized FnCas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG-FLEX.SF-iGluSnFR.A184V

   Plasmid

   #106187

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V

   Promoter

   CAG

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.SF-iGluSnFR.A184V

   Plasmid

   #106193

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V

   Promoter

   GFAP

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF2205

    Plasmid

    #144004

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

12. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_HOXB13_#2

    Plasmid

    #70094

    Purpose

    Expression of shRNA to human HOXB13, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLKO_HOXB13_#1

    Plasmid

    #70093

    Purpose

    Expression of shRNA to human HOXB13, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRS313_ATP3T911A

    Plasmid

    #120254

    Purpose

    Expression of ATP3-6 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    T911 changed to A; Isoleucine 304 changed to Aspa…

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMYC BRGI aa597-1407

    Plasmid

    #19146

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    human BRG1 aa 597-1407 (PCR product)

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.GFAP.SF-iGluSnFR.S72A

    Plasmid

    #106194

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    GFAP

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK2-E2F1 3'UTR

    Plasmid

    #29468

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F1 3'UTR (E2F1 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. psiCHECK2-BRCA1 3'UTR

    Plasmid

    #29477

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    BRCA1 3'UTR (BRCA1 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. psiCHECK2-p27Kip1 3'UTR

    Plasmid

    #29476

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    p27 Kip1 3'UTR (CDKN1B Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only