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NH2end ELL2

Plasmid

#127252

Purpose

Expresses the N terminal segment of human ELL2 in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (1-287) (ELL2 Human)

Use

Other

Tags

His6X

Expression

Mammalian

Mutation

contains only amino acids 1-289

Promoter

pEF1a

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2

Plasmid

#127268

Purpose

For in vitro translation of WT ELL2 with HA tag inserted near N-term

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

Promoter

T7

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only
pSR357.12

Plasmid

#125107

Purpose

Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)134
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Mutation

A84T - see depositor comments below and Q183K-see…

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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gh137

Plasmid

#106807

Purpose

expression of gRNA targeting EXTL1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EXTL1 (EXTL1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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CYTIP_PDZ_1

Plasmid

#103930

Purpose

Protein expression and purification of PSCDBP PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

CYTIP (CYTIP Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF37-1/2

Plasmid

#91310

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF37-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF37-1/2 (ARHGEF37 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF37-2/2

Plasmid

#91311

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF37-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF37-2/2 (ARHGEF37 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dusp5CE1-P1Egfp

Plasmid

#90151

Purpose

contains dusp5 enhancer sequence, and dusp5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5E1P1Egfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dusp5CE1-basEgfp

Plasmid

#90145

Purpose

contains dusp5 enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5E1basEgfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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PIM2 gRNA (BRDN0001149489)

Plasmid

#76384

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM2 (PIM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM2 gRNA (BRDN0001147820)

Plasmid

#76385

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM2 (PIM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK2 gRNA (BRDN0001149504)

Plasmid

#76208

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK2 (SGK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK2 gRNA (BRDN0001147898)

Plasmid

#76209

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK2 (SGK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK2 gRNA (BRDN0001145816)

Plasmid

#76206

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK2 (SGK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK2 gRNA (BRDN0001148976)

Plasmid

#76207

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK2 (SGK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK10 gRNA (BRDN0001146401)

Plasmid

#75633

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK10 (NEK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK10 gRNA (BRDN0001149193)

Plasmid

#75634

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK10 (NEK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK10 gRNA (BRDN0001162522)

Plasmid

#75635

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK10 (NEK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT6-3 (N1043S-Y1044N-Q1047E)-intein-CBD

Plasmid

#73298

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Cell. 2002 Aug 23;110(4):501-12.

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

N1043S-Y1044N-Q1047E

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pONL-C1_fibrillarin

Plasmid

#65710

Purpose

Expresses ONL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only