We narrowed to 9,129 results for: Pol

1. pGBW-m4046757

   Plasmid

   #145657

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCellFree_G03 GDI2

   Plasmid

   #67093

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GDI2 (GDI2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTRY-YFP

   Plasmid

   #15302

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YFP
   

   Use

   Gateway entry

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pbGH

   Plasmid

   #118061

   Purpose

   GBPart - Bovine growth hormone terminator

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   bgH_poly(A)
   

   Use

   Synthetic Biology; Gbpart

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIC306

   Plasmid

   #52257

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska3 (SKA3 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIA1363

   Plasmid

   #166861

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp8 (ORF1ab Synthetic)
   

   Tags

   His-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mVenus-PKCB1 K371R

   Plasmid

   #195230

   Purpose

   Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PKCB1 (PRKCB Human)
   

   Use

   Retroviral

   Mutation

   K371R

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIA1362

   Plasmid

   #166860

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp7 (ORF1ab Synthetic)
   

   Tags

   His-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBetaActin-Halo-CAMSAP3

    Plasmid

    #191329

    Purpose

    Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    Halo-CAMSAP3 (Camsap3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-Otx2-IRES-GFPd2

    Plasmid

    #73996

    Purpose

    Plasmid expressing Otx2(isoform b)-IRES-GFPd2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Otx2(isoform b)-Ires-GFP (Otx2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 human cofilin S3A

    Plasmid

    #50854

    Purpose

    expresses constituvely active cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Expression

    Mammalian

    Mutation

    constitutively active, S3A

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. 3XFlag DVL2 (∂DIX)

    Plasmid

    #24805

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of the N-terminal DIX domain

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. PB-Antares2

    Plasmid

    #120868

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-Antares2
    

    Expression

    Mammalian

    Mutation

    Antares2 amplicon

    Promoter

    CAG Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCB169

    Plasmid

    #37390

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein light chain TcTex-type 3 (DYNLT3 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 IL1B

    Plasmid

    #67066

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL1B (IL1B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. RP125 pUAST EGFP:Orco

    Plasmid

    #53441

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Orco

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Orco (Orco Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

20. C204

    Plasmid

    #174358

    Purpose

    For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Multiple silent mutations introduced to be resist…

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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