Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,609 results for: Pol

Clear keywords
Showing: 4941 - 4960 of 7609 results

  1. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

    Enlarge

  7. LEGO-GM55

    Plasmid
    #217413
    Purpose
    Luciferase expression vector with the minimal GM-CSF promoter
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    Human -55 to +28 minimal CSF2 promoter
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217413-plasmid-map-sequence-id-434968

    Enlarge

  8. pOET5 GII.4 Sydney noro-VLP

    Plasmid
    #218092
    Purpose
    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).
    Depositor
    Minna Hankaniemi
    Insert
    noro-VLP
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218092-plasmid-map-sequence-id-433318

    Enlarge

  10. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Gateway Cloning and Lentiviral
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

    Enlarge

  13. DCAF1:PBC020-D05:C246337

    Plasmid
    #183974
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Promoter
    Polyhedrin
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183974-plasmid-map-sequence-id-361427

    Enlarge

  16. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

    Enlarge

Showing: 4941 - 4960 of 7609 results