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We narrowed to 7,724 results for: Pol

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Showing: 4941 - 4960 of 7724 results

  1. AL08_pgRNA.DMD

    Plasmid
    #100293
    Purpose
    DMD intron 43-targeting gRNA expression plasmid
    Depositor
    Manuel Goncalves
    Publication
    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6 Polymerase III snRNA
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100293-plasmid-map-sequence-id-193520

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  2. AASS (AASSA-c013)

    Plasmid
    #98227
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AASSA (AASS Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Mutation
    pfam03435:Saccharop_dh 483-916
    Promoter
    polyhedrin
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98227-plasmid-map-sequence-id-193306

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  3. p5M-PR-B-1-688

    Plasmid
    #89115
    Purpose
    mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker
    Depositor
    Elizabeth Wilson
    Publication
    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6
    Insert
    human progesterone receptor-B N-term+DNA binding domain (PGR Human)
    Expression
    Mammalian
    Mutation
    5 restriction sites deleted in polylinker, contai…
    Promoter
    CMV
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89115-plasmid-map-sequence-id-175727

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  6. pTSara-R756K

    Plasmid
    #60727
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Publication
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60727-plasmid-map-sequence-id-97715

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  10. pTSlb-Q758C

    Plasmid
    #60729
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
    Depositor
    Matthew Bennett
    Publication
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60729-plasmid-map-sequence-id-99392

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  12. psicheck-2-Mut mod

    Plasmid
    #48165
    Purpose
    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element
    Depositor
    Vamsi Mootha
    Publication
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5" UTR of Mut (Mut Mouse)
    Use
    Luciferase
    Mutation
    A/T mutation in the ATG of the uORF
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. RTF1

    Plasmid
    #36198
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RTF1 (RTF1 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 347-482
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  19. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Human, Staphylococcus aureus)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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Showing: 4941 - 4960 of 7724 results