We narrowed to 9,129 results for: Pol

1. 3XFlag DVL2 (∂361-736)

   Plasmid

   #24806

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   DVL2 (DVL2 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of aa 361-736

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pBS hIhh (CT#402)

   Plasmid

   #13995

   Depositor

   Cliff Tabin

   Article

   Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

   Insert

   Ihh (IHH Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

   Plasmid

   #197968

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   C328S, C331S, L1332V

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

   Plasmid

   #204794

   Purpose

   Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   West Nile NS2B-NS3 fusion
   

   Expression

   Bacterial

   Available Since

   Aug. 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pIC292

   Plasmid

   #51405

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska2 IMAGE 4685972 (SKA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 CALR

   Plasmid

   #67086

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CALR (CALR Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pN1-CMV-L-sDarken

   Plasmid

   #184801

   Purpose

   Low affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   L-sDarken
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. px552-sg-gria1-HT-SEP

   Plasmid

   #187653

   Purpose

   Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Insert

   HaloTag-SEP donor
   

   Use

   AAV and CRISPR

   Tags

   N/A

   Promoter

   N/A

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDARMO_CMVT_N_Strep_BIRC6

   Plasmid

   #197972

   Purpose

   Mammalian cell expression of wt Strep-BIRC6

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   L1332V

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU2-A-RFP (colE1 origin)

    Plasmid

    #74093

    Purpose

    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    R0010 Const. Promoter controlling RFP

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. A3Bmax

    Plasmid

    #207167

    Purpose

    Express full-length human A3B in a BE4max-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Tags

    NLS and NLS, 6x His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU2S-b (p15A origin)

    Plasmid

    #74089

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-TRABID (NZF1, aa 1-33)

    Plasmid

    #65437

    Purpose

    Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    TRABID (ZRANB1 Human)
    

    Tags

    His6-GST

    Expression

    Bacterial

    Mutation

    only codes for amino acids 1-33 of TRABID, which …

    Promoter

    T7

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLV.PARP1#5

    Plasmid

    #14548

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4046799

    Plasmid

    #145577

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. GAL FLAG25QDProCFPp303

    Plasmid

    #15584

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pLV.PARP1#4

    Plasmid

    #14547

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

18. GAL FLAG46QDProGFP p306

    Plasmid

    #15832

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pGoldyTALEN-VIM-TAL-2

    Plasmid

    #62852

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGoldyTALEN-VIM-TAL-1

    Plasmid

    #62855

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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