We narrowed to 5,620 results for: feng zhang

1. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

   Plasmid

   #79152

   Purpose

   LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Mutation

   *

   Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0098

   Plasmid

   #142460

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MSGN1 (MSGN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF1513

   Plasmid

   #143513

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX3-1 (NKX3-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2533

   Plasmid

   #144341

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX1 (SOX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHCMV_FLUBV_HA

   Plasmid

   #207274

   Purpose

   Mammalian expression of Influenza B virus HA protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   FLUBV_HA (HA Influenza B virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF1095

   Plasmid

   #143923

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EN1 (EN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF0574

   Plasmid

   #143714

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXA13 (HOXA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF2090

   Plasmid

   #141916

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FANCD2 (FANCD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXT130_TwinStrep-SUMO-ShTnsC

    Plasmid

    #135526

    Purpose

    Protein expression construct for ShTnsC

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsC
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0630

    Plasmid

    #142668

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1I2 (NR1I2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF3054

    Plasmid

    #144530

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SALL4 (SALL4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 6His-MBP-TEV-huAsCpf1

    Plasmid

    #90095

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TwinStrep-SUMO-NLS-BhCas12b_v4

    Plasmid

    #158615

    Purpose

    For protein expression of BhCas12b_v4

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    NLS-BhCas12b_v4
    

    Use

    Tags

    6xHIS-TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF0979

    Plasmid

    #141776

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBR322-PVCpnf18-21

    Plasmid

    #198273

    Purpose

    Contains only regulatory genes; for generating empty PVCs

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf18-21
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF1761

    Plasmid

    #142843

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF24 (ZNF24 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY109 (lenti-LbCpf1)

    Plasmid

    #84740

    Purpose

    Lenti virus delivery of LbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6His-MBP-TEV-huLbCpf1

    Plasmid

    #90096

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge